PMID- 16269791
OWN - NLM
STAT- MEDLINE
DCOM- 20051220
LR  - 20190108
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 71
IP  - 11
DP  - 2005 Nov
TI  - Diversity, localization, and physiological properties of filamentous microbes 
      belonging to Chloroflexi subphylum I in mesophilic and thermophilic methanogenic 
      sludge granules.
PG  - 7493-503
AB  - We previously reported that the thermophilic filamentous anaerobe Anaerolinea 
      thermophila, which is the first cultured representative of subphylum I of the 
      bacterial phylum Chloroflexi, not only was one of the predominant constituents of 
      thermophilic sludge granules but also was a causative agent of filamentous sludge 
      bulking in a thermophilic (55 degrees C) upflow anaerobic sludge blanket (UASB) 
      reactor in which high-strength organic wastewater was treated (Y. Sekiguchi, H. 
      Takahashi, Y. Kamagata, A. Ohashi, and H. Harada, Appl. Environ. Microbiol. 
      67:5740-5749, 2001). To further elucidate the ecology and function of 
      Anaerolinea-type filamentous microbes in UASB sludge granules, we surveyed the 
      diversity, distribution, and physiological properties of Chloroflexi subphylum I 
      microbes residing in UASB granules. Five different types of mesophilic and 
      thermophilic UASB sludge were used to analyze the Chloroflexi subphylum I 
      populations. 16S rRNA gene cloning-based analyses using a 16S rRNA gene-targeted 
      Chloroflexi-specific PCR primer set revealed that all clonal sequences were 
      affiliated with the Chloroflexi subphylum I group and that a number of different 
      phylotypes were present in each clone library, suggesting the ubiquity and vast 
      genetic diversity of these populations in UASB sludge granules. Subsequent 
      fluorescence in situ hybridization (FISH) of the three different types of 
      mesophilic sludge granules using a Chloroflexi-specific probe suggested that all 
      probe-reactive cells had a filamentous morphology and were widely distributed 
      within the sludge granules. The FISH observations also indicated that the 
      Chloroflexi subphylum I bacteria were not always the predominant populations 
      within mesophilic sludge granules, in contrast to thermophilic sludge granules. 
      We isolated two mesophilic strains and one thermophilic strain belonging to the 
      Chloroflexi subphylum I group. The physiological properties of these isolates 
      suggested that these populations may contribute to the degradation of 
      carbohydrates and other cellular components, such as amino acids, in the 
      bioreactors.
FAU - Yamada, Takeshi
AU  - Yamada T
AD  - Microbial and Genetic Resources Research Group, Institute for Biological 
      Resources and Functions, National Institute of Advanced Industrial Science and 
      Technology, Central 6, 1-1 Higashi 1, Tsukuba, Ibaraki 305-8566, Japan.
FAU - Sekiguchi, Yuji
AU  - Sekiguchi Y
FAU - Imachi, Hiroyuki
AU  - Imachi H
FAU - Kamagata, Yoichi
AU  - Kamagata Y
FAU - Ohashi, Akiyoshi
AU  - Ohashi A
FAU - Harada, Hideki
AU  - Harada H
LA  - eng
SI  - GENBANK/AB109405
SI  - GENBANK/AB109406
SI  - GENBANK/AB109407
SI  - GENBANK/AB109408
SI  - GENBANK/AB109409
SI  - GENBANK/AB109410
SI  - GENBANK/AB109411
SI  - GENBANK/AB109412
SI  - GENBANK/AB109413
SI  - GENBANK/AB109414
SI  - GENBANK/AB109415
SI  - GENBANK/AB109416
SI  - GENBANK/AB109417
SI  - GENBANK/AB109418
SI  - GENBANK/AB109419
SI  - GENBANK/AB109420
SI  - GENBANK/AB109421
SI  - GENBANK/AB109422
SI  - GENBANK/AB109423
SI  - GENBANK/AB109424
SI  - GENBANK/AB109425
SI  - GENBANK/AB109426
SI  - GENBANK/AB109427
SI  - GENBANK/AB109428
SI  - GENBANK/AB109429
SI  - GENBANK/AB109430
SI  - GENBANK/AB109431
SI  - GENBANK/AB109432
SI  - GENBANK/AB109433
SI  - GENBANK/AB109434
SI  - GENBANK/AB109435
SI  - GENBANK/AB109436
SI  - GENBANK/AB109437
SI  - GENBANK/AB109438
SI  - GENBANK/AB109439
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Culture Media)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
SB  - IM
MH  - Chloroflexi/*classification/genetics/growth & development/*physiology
MH  - Culture Media
MH  - DNA, Bacterial/analysis
MH  - DNA, Ribosomal/analysis
MH  - Genes, rRNA
MH  - *Genetic Variation
MH  - Hot Temperature
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Confocal
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Sewage/*microbiology
MH  - *Temperature
PMC - PMC1287668
EDAT- 2005/11/05 09:00
MHDA- 2005/12/21 09:00
PMCR- 2005/11/01
CRDT- 2005/11/05 09:00
PHST- 2005/11/05 09:00 [pubmed]
PHST- 2005/12/21 09:00 [medline]
PHST- 2005/11/05 09:00 [entrez]
PHST- 2005/11/01 00:00 [pmc-release]
AID - 71/11/7493 [pii]
AID - 0640-05 [pii]
AID - 10.1128/AEM.71.11.7493-7503.2005 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2005 Nov;71(11):7493-503. doi: 
      10.1128/AEM.71.11.7493-7503.2005.