PMID- 16271232
OWN - NLM
STAT- MEDLINE
DCOM- 20070123
LR  - 20091119
IS  - 0529-5815 (Print)
IS  - 0529-5815 (Linking)
VI  - 43
IP  - 19
DP  - 2005 Oct 1
TI  - [Role of p38 mitogen-activated protein kinase in the pathogenesis of stress 
      ulcer].
PG  - 1284-7
AB  - OBJECTIVE: To determine whether the activation of p38 mitogen-activated protein 
      kinase (MAPK) is involved in the pathogenesis of stress ulcer. METHODS: Model of 
      stress ulcer was established with the treatment of rats with water-immersion 
      restraint (WIR) stress. Ulcer index (UI) was macroscopically evaluated as a 
      parameter of gastric mucosal lesions. Expression of phospho- and pan-p38 in 
      gastric mucosa was detected using Western blot analysis. Tumor necrosis 
      factor-alpha (TNF-alpha) and Interleukin 1beta (IL-1beta) gene expressions were 
      analyzed by Northern blot analysis. As indicated in some experiments, rats were 
      pretreated with intravenous injection of the specific p38 MAPK inhibitor CNI-1493 
      prior to WIR stress and then the changes of UI and TNF-alpha and IL-1beta mRNA 
      expression were examined. RESULTS: The p38 MAPK was persistently activated in the 
      gastric mucosa of rats with WIR stress, with maximal activation after 1 h of 
      stress [(6.8 +/- 3.2) fold of baseline levels, P < 0.01]. Inhibition of p38 MAPK 
      activation with CNI-1493 led to a marked decrease in UI in WIR stress rats. 
      Similarly, the increased gene expression of proinflammatory cytokines TNF-alpha 
      and IL-1beta in gastric mucosa induced by WIR stress were significantly 
      diminished by p38 MAPK inhibition. CONCLUSION: p38 MAPK might have an important 
      role in the pathogenesis of stress ulcer.
FAU - Jia, Yi-tao
AU  - Jia YT
AD  - Department of Burns, Changhai Hospital, Second Military Medical University, 
      Shanghai 200433, China.
FAU - Wei, Duo
AU  - Wei D
FAU - Xia, Zhao-fan
AU  - Xia ZF
FAU - Tian, Jian-guang
AU  - Tian JG
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Wai Ke Za Zhi
JT  - Zhonghua wai ke za zhi [Chinese journal of surgery]
JID - 0153611
RN  - 0 (Interleukin-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Interleukin-1/genetics
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stomach Ulcer/enzymology/*etiology/genetics
MH  - Stress, Physiological/*complications
MH  - Tumor Necrosis Factor-alpha/genetics
MH  - p38 Mitogen-Activated Protein Kinases/metabolism/*physiology
EDAT- 2005/11/08 09:00
MHDA- 2007/01/24 09:00
CRDT- 2005/11/08 09:00
PHST- 2005/11/08 09:00 [pubmed]
PHST- 2007/01/24 09:00 [medline]
PHST- 2005/11/08 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Wai Ke Za Zhi. 2005 Oct 1;43(19):1284-7.