PMID- 16271951
OWN - NLM
STAT- MEDLINE
DCOM- 20060123
LR  - 20061115
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 163
IP  - 1
DP  - 2005 Nov
TI  - Characterization of the TSU-PR1 cell line by chromosome painting and flow 
      cytometry.
PG  - 17-22
AB  - TSU-PR1 was originally reported as a prostatic carcinoma cell line derived from a 
      lymph node metastasis. Recently, however, this cell line was reported to be 
      derived from T24 bladder carcinoma cells, and thus further definition of its 
      origin is needed. Conventional cytogenetic study of TSU-PR1 showed aneuploidy, 
      ranging from 65 to 86 chromosome with a modal number of 80, and with 10 marker 
      chromosomes, thus conventional cytogenetics cannot be used to determine which 
      chromosomes or regions of chromosomes are critical in cancer development and 
      progression of this cell line. The present study was conducted to characterize 
      genetic changes of the cell line using comparative genomic hybridization (CGH), 
      fluorescence in situ hybridization (FISH), and flow cytometry. CGH results showed 
      that green-to-red fluorescence ratios were within the range of 0.85-1.15, except 
      for a few chromosomes, which reflected near tetraploidy in TSU-PR1. Flow 
      cytometric analysis of TSU-PR1 revealed a DNA index of 3.46n, which is close to 
      the 3.48n calculated from a modal number of 80. The copy numbers of chromosomes 
      4, 6, 7, 17, and 20 determined by the DNA index and the CGH analyses were 2.85 
      +/- 0.09, 3.22 +/- 0.77, 3.01 +/- 0.26, 4.05 +/- 0.44, and 4.99 +/- 0.48, 
      respectively. These numbers are also in accordance with the chromosome copy 
      numbers determined with FISH: 2.98 +/- 0.23, 2.91 +/- 0.44, 2.74 +/- 0.44, 3.93 
      +/- 0.38, and 5.05 +/- 0.78 for chromosomes 4, 6, 7, 17, and 20, respectively (P 
      > 0.05).
FAU - Kim, M S
AU  - Kim MS
AD  - Dental Science Research Institute, Department of Oral Anatomy, School of 
      Dentistry, Chonnam National University, Yongbongdong, Gwangju 500-757, Republic 
      of Korea.
FAU - Kim, S H
AU  - Kim SH
FAU - Kim, H J
AU  - Kim HJ
FAU - Hoang, I N
AU  - Hoang IN
FAU - Oh, W M
AU  - Oh WM
FAU - Koh, J T
AU  - Koh JT
FAU - Park, H O
AU  - Park HO
FAU - Jeong, J Y
AU  - Jeong JY
FAU - Kim, W J
AU  - Kim WJ
FAU - Lee, E J
AU  - Lee EJ
FAU - Koh, J Y
AU  - Koh JY
FAU - Kim, B Y
AU  - Kim BY
FAU - Jensen, R H
AU  - Jensen RH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Carcinoma/genetics
MH  - Cell Line, Tumor
MH  - Chromosome Mapping
MH  - Chromosome Painting/*methods
MH  - Flow Cytometry/methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes/cytology/pathology
MH  - Male
MH  - Metaphase
MH  - Nucleic Acid Hybridization
MH  - Prostatic Neoplasms/*genetics/pathology
EDAT- 2005/11/08 09:00
MHDA- 2006/01/24 09:00
CRDT- 2005/11/08 09:00
PHST- 2005/01/11 00:00 [received]
PHST- 2005/04/07 00:00 [revised]
PHST- 2005/04/08 00:00 [accepted]
PHST- 2005/11/08 09:00 [pubmed]
PHST- 2006/01/24 09:00 [medline]
PHST- 2005/11/08 09:00 [entrez]
AID - S0165-4608(05)00217-7 [pii]
AID - 10.1016/j.cancergencyto.2005.04.010 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2005 Nov;163(1):17-22. doi: 
      10.1016/j.cancergencyto.2005.04.010.