PMID- 16271952
OWN - NLM
STAT- MEDLINE
DCOM- 20060123
LR  - 20071114
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 163
IP  - 1
DP  - 2005 Nov
TI  - Molecular characterization of the t(3;9) associated with immortalization in the 
      MCF10A cell line.
PG  - 23-9
AB  - The t(3;9)(p14;p21) in the MCF10A human mammary gland epithelial cell line was 
      the single cytogenetic event that accompanied the transition from primary culture 
      to immortalized cell line, suggesting that it is related to the development of 
      the immortalization phenotype. To study the molecular consequences of the 
      breakpoints in this rearrangement, we used a combination of fluorescence in situ 
      hybridization (FISH) and array comparative genomic hybridization (CGH). The 3p14 
      translocation breakpoint occurs within BAC RP11-795e22, which accommodates only 
      the TAFA1 gene, a novel cysteine-rich secreted protein thought to be involved in 
      cytokine signaling. TAFA1 is expressed in normal breast tissue, not in MCF10A, 
      and shows differential expression in a range of breast cancer cell lines. The 9p 
      translocation breakpoint results in a deletion of approximately 4 megabases on 
      the derivative chromosome 9, which includes the CDKN2A (p16) gene. Array CGH and 
      FISH analysis demonstrated that BAC 149i22, which contains the CDKN2A/B genes, is 
      also deleted specifically on the apparently normal copy of chromosome 9, making 
      MCF10A null for the p16/p15 genes. The exact extent of gains and losses of 
      chromosome regions resulting from rearrangements involving chromosomes 1q, 5q, 
      and 8q have also been characterized using the BAC arrays.
FAU - Cowell, John K
AU  - Cowell JK
AD  - Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, NY 14263, 
      USA. John.Cowell@RoswellPark.org
FAU - LaDuca, Jeffrey
AU  - LaDuca J
FAU - Rossi, Michael R
AU  - Rossi MR
FAU - Burkhardt, Tania
AU  - Burkhardt T
FAU - Nowak, Norma J
AU  - Nowak NJ
FAU - Matsui, Sei-ichi
AU  - Matsui S
LA  - eng
GR  - CA 16056/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Cell Line, Tumor
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 3
MH  - *Chromosomes, Human, Pair 9
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Mammary Glands, Human/cytology/*physiology
MH  - Nucleic Acid Hybridization
MH  - Oligonucleotide Array Sequence Analysis
MH  - Phenotype
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Translocation, Genetic
EDAT- 2005/11/08 09:00
MHDA- 2006/01/24 09:00
CRDT- 2005/11/08 09:00
PHST- 2005/04/08 00:00 [received]
PHST- 2005/04/27 00:00 [revised]
PHST- 2005/04/28 00:00 [accepted]
PHST- 2005/11/08 09:00 [pubmed]
PHST- 2006/01/24 09:00 [medline]
PHST- 2005/11/08 09:00 [entrez]
AID - S0165-4608(05)00237-2 [pii]
AID - 10.1016/j.cancergencyto.2005.04.019 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2005 Nov;163(1):23-9. doi: 
      10.1016/j.cancergencyto.2005.04.019.