PMID- 16273599
OWN - NLM
STAT- MEDLINE
DCOM- 20060320
LR  - 20190430
IS  - 1007-9327 (Print)
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Linking)
VI  - 11
IP  - 38
DP  - 2005 Oct 14
TI  - Inhibitory effects of extracellular adenosine triphosphate on growth of 
      esophageal carcinoma cells.
PG  - 5915-9
AB  - AIM: To study the growth inhibitory effects of ATP on TE-13 human squamous 
      esophageal carcinoma cells in vitro. METHODS: MTT assay was used to determine the 
      inhibition of proliferation of ATP or adenosine (ADO) on TE-13 cell line. The 
      morphological changes of TE-13 cells induced by ATP or ADO were observed under 
      fluorescence light microscope by acridine orange (AO)/ethidium bromide (EB) 
      double stained cells. The internucleosomal fragmentation of genomic DNA was 
      detected by agarose gel electrophoresis. The apoptotic rate and cell cycle after 
      treatment with ATP or ADO were determined by flow cytometry. RESULTS: ATP and ADO 
      produced inhibitory effects on TE-13 cells at the concentration between 0.01 and 
      1.0 mmol/L. The IC(50) of TE-13 cells exposed to ATP or ADO for 48 and 72 h was 
      0.71 or 1.05, and 0.21 or 0.19 mmol/L, respectively. The distribution of cell 
      cycle phase and proliferation index (PI) value of TE-13 cells changed, when being 
      exposed to ATP or ADO at the concentrations of 0.01, 0.1, and 1 mmol/L for 48 h. 
      ATP and ADO inhibited the cell proliferation by changing the distribution of cell 
      cycle phase via either G(0)/G(1) phase (ATP or ADO, 1 mmol/L) or S phase (ATP, 
      0.1 mmol/L) arrest. Under light microscope, the tumor cells exposed to 0.3 mmol/L 
      ATP or ADO displayed morphological changes of apoptosis. A ladder-like pattern of 
      DNA fragmentation was obtained from TE-13 cells treated with 0.1-1 mmol/L ATP or 
      ADO in agarose gel electrophoresis. ATP and ADO induced apoptosis of TE-13 cells 
      in a dose-dependent manner at the concentration between 0.03 and 1 mmol/L. The 
      maximum apoptotic rate of TE-13 cells exposed to ATP or ADO for 48 h was 16.63% 
      or 16.9%, respectively. CONCLUSION: ATP and ADO inhibit cell proliferation, 
      arrest cell cycle, and induce apoptosis of TE-13 cell line.
FAU - Wang, Ming-Xia
AU  - Wang MX
AD  - School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, Hebei 
      Province, China.
FAU - Ren, Lei-Ming
AU  - Ren LM
FAU - Shan, Bao-En
AU  - Shan BE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - K72T3FS567 (Adenosine)
SB  - IM
MH  - Adenosine/pharmacology
MH  - Adenosine Triphosphate/*pharmacology
MH  - Apoptosis/drug effects
MH  - Cell Cycle/drug effects
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - DNA Fragmentation/drug effects
MH  - Esophageal Neoplasms/*drug therapy/metabolism/pathology
MH  - Humans
PMC - PMC4436710
EDAT- 2005/11/08 09:00
MHDA- 2006/03/21 09:00
PMCR- 2005/10/14
CRDT- 2005/11/08 09:00
PHST- 2005/11/08 09:00 [pubmed]
PHST- 2006/03/21 09:00 [medline]
PHST- 2005/11/08 09:00 [entrez]
PHST- 2005/10/14 00:00 [pmc-release]
AID - 10.3748/wjg.v11.i38.5915 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2005 Oct 14;11(38):5915-9. doi: 10.3748/wjg.v11.i38.5915.