PMID- 16274303
OWN - NLM
STAT- MEDLINE
DCOM- 20060207
LR  - 20220409
IS  - 0022-3492 (Print)
IS  - 0022-3492 (Linking)
VI  - 76
IP  - 11
DP  - 2005 Nov
TI  - Gingival crevicular fluid levels of monocyte chemoattractant protein-1 and tumor 
      necrosis factor-alpha in patients with chronic and aggressive periodontitis.
PG  - 1849-55
AB  - BACKGROUND: Monocyte chemoattractant protein-1 (MCP-1) is a well-known 
      chemotactic cytokine that regulates mononuclear inflammatory cell recruitment. 
      This recruitment has particular importance in the oral cavity because 
      inflammatory cells will be challenged with periodontopathogenic bacteria during 
      infections. Tumor necrosis factor-alpha (TNF-alpha) is a cytokine that induces 
      bone resorption by stimulating proliferation and differentiation of osteoclasts' 
      progenitors and also stimulates MCP-1 expression. The aims of this study were to 
      investigate the presence of MCP-1 in gingival crevicular fluid (GCF) samples from 
      patients with chronic periodontitis (CP) and aggressive periodontitis (AgP) and 
      to examine the possible correlations between the GCF levels of MCP-1 and 
      TNF-alpha. METHODS: Twenty-five CP, 20 AgP, and 20 healthy control (C) patients 
      were selected for the study. Plaque index (PI), gingival index (GI), probing 
      depth (PD), and clinical attachment level (CAL) measurements were recorded from 
      each sampling area. Assays for GCF MCP-1 and TNF-alpha were carried out by an 
      enzyme-linked immunosorbent assay (ELISA) method. RESULTS: The concentration 
      (nanograms per microliters) and total MCP-1 and TNF-alpha (nanograms per site) 
      were not statistically significant between CP and AgP groups, but total MCP-1 and 
      TNF-alpha was statistically different between CP and C and between AgP and C 
      groups (P <0.001). All clinical parameters were statistically different between 
      CP and C and between AgP and C groups (P <0.001). A positive statistical 
      correlation was detected between the levels of MCP-1 and TNF-alpha, and there was 
      also a positive correlation between all clinical parameters and total MCP-1 and 
      TNF-alpha levels. CONCLUSIONS: These results suggest that MCP-1 could have an 
      important role in the activation and recruitment of inflammatory and immune cells 
      in periodontal diseases, and both AgP and CP patients may have the same pattern 
      of MCP-1 expression. A strong positive correlation between the GCF levels of 
      MCP-1 and TNF-alpha may account for the mechanism of amplification of 
      inflammatory events in gingival inflammation.
FAU - Kurtis, Bulent
AU  - Kurtis B
AD  - Department of Periodontology, Faculty of Dentistry, Gazi University, Ankara, 
      Turkey. bulkurtis@hotmail.com
FAU - Tuter, Gulay
AU  - Tuter G
FAU - Serdar, Muhittin
AU  - Serdar M
FAU - Akdemir, Pelin
AU  - Akdemir P
FAU - Uygur, Cem
AU  - Uygur C
FAU - Firatli, Erhan
AU  - Firatli E
FAU - Bal, Belgin
AU  - Bal B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Periodontol
JT  - Journal of periodontology
JID - 8000345
RN  - 0 (Chemokine CCL2)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Chemokine CCL2/*analysis
MH  - Chronic Disease
MH  - Dental Plaque Index
MH  - Female
MH  - Gingival Crevicular Fluid/*immunology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Periodontal Attachment Loss/classification/immunology
MH  - Periodontal Index
MH  - Periodontal Pocket/classification/immunology
MH  - Periodontitis/classification/*immunology
MH  - Tumor Necrosis Factor-alpha/*analysis
EDAT- 2005/11/09 09:00
MHDA- 2006/02/08 09:00
CRDT- 2005/11/09 09:00
PHST- 2005/11/09 09:00 [pubmed]
PHST- 2006/02/08 09:00 [medline]
PHST- 2005/11/09 09:00 [entrez]
AID - 10.1902/jop.2005.76.11.1849 [doi]
PST - ppublish
SO  - J Periodontol. 2005 Nov;76(11):1849-55. doi: 10.1902/jop.2005.76.11.1849.