PMID- 16275225
OWN - NLM
STAT- MEDLINE
DCOM- 20060118
LR  - 20071115
IS  - 1556-5653 (Electronic)
IS  - 0015-0282 (Linking)
VI  - 84
IP  - 5
DP  - 2005 Nov
TI  - Self-correction of chromosomally abnormal embryos in culture and implications for 
      stem cell production.
PG  - 1328-34
AB  - OBJECTIVE: To ascertain whether embryos classified by preimplantation genetic 
      diagnosis (PGD) for infertility as abnormal and then plated to obtain stem cells 
      would self-correct partially or totally in culture, producing disomic stem cells. 
      DESIGN: Prospective study to determine the chromosome status of embryos on day 3 
      and 6, as well as cultured cells derived from inner cell masses from the same 
      embryos when cultured up to day 12. SETTING: Research laboratory. PATIENT(S): 
      Patients undergoing PGD of aneuploidy. INTERVENTION(S): Of 142 embryos classified 
      by PGD for aneuploidy as abnormal, 50 were cultured to the blastocyst stage. At 
      that stage a fraction of the embryos underwent trophectoderm biopsy to reconfirm 
      the PGD diagnosis. After further co-culture with feeders up to day 12, 34 embryos 
      attached to the feeder cells. Of those, 24 were analyzed by fluorescence in situ 
      hybridization (FISH) and the rest for the expression of Oct-4, SSEA-3, SSEA-4, 
      TRA1-60, and TRA1-80. MAIN OUTCOME MEASURE(S): Disomic cells obtained from 
      trisomic embryos. RESULT(S): Analysis by FISH of day-12 cultures showed that 7 
      were totally normal, 6 were mostly abnormal, and 11 had experienced some 
      chromosome normalization, having between 21% and 88% normal cells. Day-12 culture 
      was positive for Oct-4 expression by reverse transcriptase polymerase chain 
      reaction analysis and for SSEA-3, SSEA-4, TRA1-60, and TRA1-80 by 
      immunocytochemistry. CONCLUSION(S): Chromosome self-normalization occurs in a 
      significant proportion of chromosomally abnormal embryos, possibly because of the 
      loss of a chromosome in trisomic cells after blastocyst stage. Thus chromosomally 
      abnormal embryos are a potential source of disomic stem cells. Not all 
      chromosomally abnormal embryos self-corrected. Abnormal stem cells that might be 
      derived could be used as models to study the effect of chromosomal abnormalities 
      on human development.
FAU - Munne, Santiago
AU  - Munne S
AD  - Reprogenetics, LLC, West Orange, New Jersey 07052, USA. munne@embryos.net
FAU - Velilla, Esther
AU  - Velilla E
FAU - Colls, Pere
AU  - Colls P
FAU - Garcia Bermudez, Mercedez
AU  - Garcia Bermudez M
FAU - Vemuri, Mohan C
AU  - Vemuri MC
FAU - Steuerwald, Nury
AU  - Steuerwald N
FAU - Garrisi, John
AU  - Garrisi J
FAU - Cohen, Jacques
AU  - Cohen J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
SB  - IM
MH  - Chromosome Aberrations/*embryology
MH  - *Embryo Culture Techniques/statistics & numerical data
MH  - Embryo, Mammalian/cytology/*physiology
MH  - Humans
MH  - Preimplantation Diagnosis/methods/statistics & numerical data
MH  - Prospective Studies
MH  - Stem Cells/cytology/*physiology
EDAT- 2005/11/09 09:00
MHDA- 2006/01/19 09:00
CRDT- 2005/11/09 09:00
PHST- 2005/02/07 00:00 [received]
PHST- 2005/06/30 00:00 [revised]
PHST- 2005/06/30 00:00 [accepted]
PHST- 2005/11/09 09:00 [pubmed]
PHST- 2006/01/19 09:00 [medline]
PHST- 2005/11/09 09:00 [entrez]
AID - S0015-0282(05)02941-9 [pii]
AID - 10.1016/j.fertnstert.2005.06.025 [doi]
PST - ppublish
SO  - Fertil Steril. 2005 Nov;84(5):1328-34. doi: 10.1016/j.fertnstert.2005.06.025.