PMID- 16275234
OWN - NLM
STAT- MEDLINE
DCOM- 20060118
LR  - 20061115
IS  - 1556-5653 (Electronic)
IS  - 0015-0282 (Linking)
VI  - 84
IP  - 5
DP  - 2005 Nov
TI  - Fluorescence in situ hybridization reanalysis of day-6 human blastocysts 
      diagnosed with aneuploidy on day 3.
PG  - 1395-400
AB  - OBJECTIVE: To determine the concordance of day-6 blastocyst analysis with the 
      day-3 fluorescence in situ hybridization (FISH) aneuploidy diagnosis. DESIGN: 
      Retrospective study. SETTING: In vitro fertilization laboratory. PATIENT(S): Six 
      hundred sixty embryos were included from 94 IVF/intracytoplasmic sperm injection 
      patients undergoing preimplantation genetic diagnosis. INTERVENTION(S): None. 
      MAIN OUTCOME MEASURE(S): Single blastomeres biopsied on day 3. Aneuploidy 
      screening for chromosomes 13, 18, 21, X, and Y were analyzed. Left-over 
      blastocysts were reanalyzed on day 6. RESULT(S): Among the 660 embryos evaluated, 
      367 (55.6%) were euploid and 281 (42.6%) were aneuploid. Of the euploid embryos, 
      213 embryos were transferred, 68 were frozen on day 5, and 86 were left. All 281 
      aneuploid embryos were further cultured, and 55 (19.6%) progressed to 
      blastocysts. When FISH reanalysis was performed, 33 of 55 blastocysts (60%) were 
      confirmed aneuploid in concordance with the day-3 diagnosis. However, 22 of 55 
      blastocysts (40%) were determined to be euploid. In addition, 207 aneuploid 
      embryos (73.7%) arrested before day 6, as opposed to 32 of the 86 euploid embryos 
      (37.2%). CONCLUSION(S): Day-3 single-cell embryo biopsy reveals that aneuploidy 
      can be confirmed in 60.7% of the blastocysts on reanalysis. The majority of 
      discordance is most likely due to embryo mosaicism and possibly a limited ability 
      to "self-correct."
FAU - Li, Man
AU  - Li M
AD  - ART Reproductive Center, Beverly Hills, California 90210, USA. 
      mli@artreproductivecenter.com
FAU - DeUgarte, Catherine Marin
AU  - DeUgarte CM
FAU - Surrey, Mark
AU  - Surrey M
FAU - Danzer, Hal
AU  - Danzer H
FAU - DeCherney, Alan
AU  - DeCherney A
FAU - Hill, David L
AU  - Hill DL
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
SB  - IM
CIN - Fertil Steril. 2006 Apr;85(4):e3; author reply e4. PMID: 16580349
CIN - Fertil Steril. 2006 Sep;86(3):768; author reply 768-9. PMID: 16952516
MH  - *Aneuploidy
MH  - Blastocyst/*physiology
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods/*standards
MH  - Preimplantation Diagnosis/*methods/*standards
MH  - Reproducibility of Results
MH  - Retrospective Studies
EDAT- 2005/11/09 09:00
MHDA- 2006/01/19 09:00
CRDT- 2005/11/09 09:00
PHST- 2004/08/01 00:00 [received]
PHST- 2005/04/25 00:00 [revised]
PHST- 2005/04/25 00:00 [accepted]
PHST- 2005/11/09 09:00 [pubmed]
PHST- 2006/01/19 09:00 [medline]
PHST- 2005/11/09 09:00 [entrez]
AID - S0015-0282(05)02928-6 [pii]
AID - 10.1016/j.fertnstert.2005.04.068 [doi]
PST - ppublish
SO  - Fertil Steril. 2005 Nov;84(5):1395-400. doi: 10.1016/j.fertnstert.2005.04.068.