PMID- 16275755
OWN - NLM
STAT- MEDLINE
DCOM- 20060124
LR  - 20181113
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 171
IP  - 3
DP  - 2005 Nov 7
TI  - Temporally resolved interactions between antigen-stimulated IgE receptors and Lyn 
      kinase on living cells.
PG  - 527-36
AB  - Upon cross-linking by antigen, the high affinity receptor for immunoglobulin E 
      (IgE), FcepsilonRI, is phosphorylated by the Src family tyrosine kinase Lyn to 
      initiate mast cell signaling, leading to degranulation. Using fluorescence 
      correlation spectroscopy (FCS), we observe stimulation-dependent associations 
      between fluorescently labeled IgE-FcepsilonRI and Lyn-EGFP on individual cells. 
      We also simultaneously measure temporal variations in the lateral diffusion of 
      these proteins. Antigen-stimulated interactions between these proteins detected 
      subsequent to the initiation of receptor phosphorylation exhibit time-dependent 
      changes, suggesting multiple associations between FcepsilonRI and Lyn-EGFP. 
      During this period, we also observe a persistent decrease in Lyn-EGFP lateral 
      diffusion that is dependent on Src family kinase activity. These stimulated 
      interactions are not observed between FcepsilonRI and a chimeric EGFP that 
      contains only the membrane-targeting sequence from Lyn. Our results reveal 
      real-time interactions between Lyn and cross-linked FcepsilonRI implicated in 
      downstream signaling events. They demonstrate the capacity of FCS 
      cross-correlation analysis to investigate the mechanism of signaling-dependent 
      protein-protein interactions in intact, living cells.
FAU - Larson, Daniel R
AU  - Larson DR
AD  - School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, 
      USA.
FAU - Gosse, Julie A
AU  - Gosse JA
FAU - Holowka, David A
AU  - Holowka DA
FAU - Baird, Barbara A
AU  - Baird BA
FAU - Webb, Watt W
AU  - Webb WW
LA  - eng
GR  - P41 EB001976/EB/NIBIB NIH HHS/United States
GR  - R01 AI018306/AI/NIAID NIH HHS/United States
GR  - 9 P41 EB001976/EB/NIBIB NIH HHS/United States
GR  - AI18306/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Receptors, IgE)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (enhanced green fluorescent protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 2.7.10.2 (lyn protein-tyrosine kinase)
RN  - EC 2.7.10.2 (src-Family Kinases)
SB  - IM
CIN - J Cell Biol. 2005 Nov 7;171(3):415-7. PMID: 16275748
MH  - Animals
MH  - Cell Degranulation
MH  - Cell Line
MH  - Cell Membrane/*metabolism
MH  - Diffusion
MH  - Green Fluorescent Proteins/genetics
MH  - Humans
MH  - Immunoglobulin E/*physiology
MH  - Mast Cells/physiology
MH  - Membrane Microdomains/physiology
MH  - Mice
MH  - Phosphorylation
MH  - Rats
MH  - Receptors, IgE/*metabolism
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Signal Transduction
MH  - Spectrometry, Fluorescence
MH  - src-Family Kinases/antagonists & inhibitors/genetics/*metabolism
PMC - PMC2171255
EDAT- 2005/11/09 09:00
MHDA- 2006/01/25 09:00
PMCR- 2006/05/07
CRDT- 2005/11/09 09:00
PHST- 2005/11/09 09:00 [pubmed]
PHST- 2006/01/25 09:00 [medline]
PHST- 2005/11/09 09:00 [entrez]
PHST- 2006/05/07 00:00 [pmc-release]
AID - jcb.200503110 [pii]
AID - 200503110 [pii]
AID - 10.1083/jcb.200503110 [doi]
PST - ppublish
SO  - J Cell Biol. 2005 Nov 7;171(3):527-36. doi: 10.1083/jcb.200503110.