PMID- 16275896
OWN - NLM
STAT- MEDLINE
DCOM- 20060302
LR  - 20181203
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 79
IP  - 1
DP  - 2006 Jan
TI  - Prostaglandin E2 promotes degranulation-independent release of MCP-1 from mast 
      cells.
PG  - 95-104
AB  - Mast cells (MCs) are common components of inflammatory infiltrates and a source 
      of proangiogenic factors. Inflammation is often accompanied by vascular changes. 
      However, little is known about modulation of MC-derived proangiogenic factors 
      during inflammation. In this study, we evaluated the effects of the 
      proinflammatory mediator prostaglandin E2 (PGE2) on MC expression and release of 
      proangiogenic factors. We report that PGE2 dose-dependently induces primary MCs 
      to release the proangiogenic chemokine monocyte chemoattractant protein-1 
      (MCP-1). This release of MCP-1 is complete by 2 h after PGE2 exposure, reaches 
      levels of MCP-1 at least 15-fold higher than background, and is not accompanied 
      by degranulation or increased MCP-1 gene expression. By immunoelectron 
      microscopy, MCP-1 is detected within MCs at a cytoplasmic location distinct from 
      the secretory granules. Dexamethasone and cyclosporine A inhibit PGE2-induced 
      MCP-1 secretion by approximately 60%. Agonists of PGE2 receptor subtypes revealed 
      that the EP1 and EP3 receptors can independently mediate MCP-1 release from MCs. 
      These observations identify PGE2-induced MCP-1 release from MCs as a pathway 
      underlying inflammation-associated angiogenesis and extend current understanding 
      of the activities of PGE2.
FAU - Nakayama, Takayuki
AU  - Nakayama T
AD  - Experimental Transplantation and Immunology Branch, Center for Cancer Research, 
      National Cancer Institute, Bethesda, Maryland 20892, USA. Nakayamt@mail.nih.gov
FAU - Mutsuga, Noriko
AU  - Mutsuga N
FAU - Yao, Lei
AU  - Yao L
FAU - Tosato, Giovanna
AU  - Tosato G
LA  - eng
GR  - Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20051107
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Angiogenesis Inducing Agents)
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (Oxytocics)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 83HN0GTJ6D (Cyclosporine)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Angiogenesis Inducing Agents/immunology/*metabolism
MH  - Animals
MH  - Anti-Inflammatory Agents/pharmacology
MH  - Cell Degranulation/*drug effects/immunology
MH  - Cells, Cultured
MH  - Chemokine CCL2/immunology/*metabolism
MH  - Cyclosporine/pharmacology
MH  - Dexamethasone/pharmacology
MH  - Dinoprostone/immunology/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Drug Antagonism
MH  - Immunosuppressive Agents/pharmacology
MH  - Inflammation/immunology/metabolism/pathology
MH  - Mast Cells/immunology/*metabolism/ultrastructure
MH  - Mice
MH  - Neovascularization, Pathologic/immunology/pathology
MH  - Oxytocics/immunology/*pharmacology
MH  - Secretory Vesicles/immunology/metabolism/ultrastructure
EDAT- 2005/11/09 09:00
MHDA- 2006/03/03 09:00
CRDT- 2005/11/09 09:00
PHST- 2005/11/09 09:00 [pubmed]
PHST- 2006/03/03 09:00 [medline]
PHST- 2005/11/09 09:00 [entrez]
AID - jlb.0405226 [pii]
AID - 10.1189/jlb.0405226 [doi]
PST - ppublish
SO  - J Leukoc Biol. 2006 Jan;79(1):95-104. doi: 10.1189/jlb.0405226. Epub 2005 Nov 7.