PMID- 16276101
OWN - NLM
STAT- MEDLINE
DCOM- 20060203
LR  - 20061115
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 112
IP  - 1-2
DP  - 2006
TI  - Further insights into the ancestral murine karyotype: the contribution of the 
      Otomys-Mus comparison using chromosome painting.
PG  - 126-30
AB  - The African vlei rat, Otomys irroratus, comprises several distinct chromosomal 
      races that may be grouped into two major cytogenetic clades. Recognition of these 
      clades is underpinned by a complex chromosomal rearrangement involving three 
      different autosomes in the unfused state. We have used unidirectional 
      fluorescence in situ hybridization (FISH) of mouse chromosome-specific painting 
      probes to molecularly define the components of this rearrangement as well as to 
      establish the chromosomal homologies between the mouse and the vlei rat genomes. 
      This has allowed for the detection of 41 autosomal segments of conserved synteny. 
      Nine mouse chromosomes were conserved in toto (MMU3, 4, 6, 7, 11, 12, 14, 18, 19) 
      with a further seven (MMU2, 5, 8, 9, 10, 13, 16) showing homology to two discrete 
      regions in the vlei rat genome. Two mouse autosomes (MMU15, 17) correspond to 
      three regions in O. irroratus with MMU1 being the most fragmented showing five 
      sites of hybridization in this species. By mapping these data to published 
      sequence-based phylogenies we are able to confirm most of the published putative 
      ancestral murine chromosomal states. Our data further indicate that MMU15a+ 
      MMU13b+MMU10b+MMU17b was present in the murine ancestral karyotype suggesting an 
      ancestral 2n = 52 rather than the 2n = 54 previously postulated.
CI  - Copyright 2006 S. Karger AG, Basel.
FAU - Engelbrecht, A
AU  - Engelbrecht A
AD  - Department of Botany and Zoology, Stellenbosch University, Matieland, South 
      Africa.
FAU - Dobigny, G
AU  - Dobigny G
FAU - Robinson, T J
AU  - Robinson TJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Chromosome Mapping
MH  - *Chromosome Painting
MH  - *Evolution, Molecular
MH  - Fibroblasts/cytology/physiology
MH  - Gene Rearrangement
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Mice/*genetics
MH  - Muridae/*genetics
EDAT- 2005/11/09 09:00
MHDA- 2006/02/04 09:00
CRDT- 2005/11/09 09:00
PHST- 2005/04/12 00:00 [received]
PHST- 2005/04/28 00:00 [accepted]
PHST- 2005/11/09 09:00 [pubmed]
PHST- 2006/02/04 09:00 [medline]
PHST- 2005/11/09 09:00 [entrez]
AID - 87524 [pii]
AID - 10.1159/000087524 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2006;112(1-2):126-30. doi: 10.1159/000087524.