PMID- 16276106
OWN - NLM
STAT- MEDLINE
DCOM- 20060203
LR  - 20061115
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 112
IP  - 1-2
DP  - 2006
TI  - Molecular cytogenetic characterization and chromosomal distribution of the 
      satellite DNA in the genome of Oxya hyla intricata (Orthoptera: Catantopidae).
PG  - 160-5
AB  - The genomic DNA of the grasshopper (Oxya hyla intricata) was subjected to 
      electrophoresis after digestion with HaeIII, and the result showed two bands of 
      highly repetitive DNA, approximately 200 and 400 bp in length. The 200-bp 
      HaeIII-digested fragment was cloned and characterized by sequencing and 
      fluorescence in situ hybridization (FISH). The results showed the presence of two 
      distinct satellite DNA (stDNA) families: one consisting of a 169-bp repeated 
      element having an A+T content of 60.9% and the other consisting of a 204-bp 
      repeated element having an A+T content of 53.9%. No significant homology between 
      the two stDNA families was observed. FISH showed that the chromosomal locations 
      of these families are different from each other. The 169-bp element was located 
      in the C-band-positive regions of the short arms of most of the chromosomes, 
      whereas the 204-bp element was located in the centromeric regions of three 
      chromosome pairs. These results imply that the origins of these two DNA families 
      are different. The results of zoo-blot hybridization to the genomic DNA from four 
      Oxya species, O. hyla intricata, O. japonica japonica, O. chinensis formosana, 
      and O. yezoensis, suggest that the two stDNA families found in the present study 
      are species-specific for O. hyla intricata.
CI  - Copyright 2006 S. Karger AG, Basel.
FAU - Yoshimura, A
AU  - Yoshimura A
AD  - Department of Biofunctional Science, Faculty of Agriculture and Life Science, 
      Hirosaki University, Hirosaki, Japan. ayoshimura8@hotmail.com
FAU - Nakata, A
AU  - Nakata A
FAU - Kuro-o, M
AU  - Kuro-o M
FAU - Obara, Y
AU  - Obara Y
FAU - Ando, Y
AU  - Ando Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (DNA, Satellite)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Banding
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - Consensus Sequence
MH  - Cytogenetics/methods
MH  - DNA/genetics
MH  - DNA, Satellite/*genetics
MH  - Grasshoppers/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Orthoptera/genetics
MH  - Sequence Alignment
MH  - Sequence Homology, Nucleic Acid
MH  - Species Specificity
EDAT- 2005/11/09 09:00
MHDA- 2006/02/04 09:00
CRDT- 2005/11/09 09:00
PHST- 2004/12/30 00:00 [received]
PHST- 2005/03/01 00:00 [accepted]
PHST- 2005/11/09 09:00 [pubmed]
PHST- 2006/02/04 09:00 [medline]
PHST- 2005/11/09 09:00 [entrez]
AID - 87529 [pii]
AID - 10.1159/000087529 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2006;112(1-2):160-5. doi: 10.1159/000087529.