PMID- 16276865
OWN - NLM
STAT- MEDLINE
DCOM- 20090414
LR  - 20131121
IS  - 0001-6209 (Print)
IS  - 0001-6209 (Linking)
VI  - 43
IP  - 1
DP  - 2003 Feb
TI  - [Fusion expression of the ORF5 gene of porcine reproductive and respiratory 
      syndrome virus in insect cells].
PG  - 8-14
AB  - A 0.75kb fragment containing the complete cDNA of glutathione-S-transferase (GST) 
      and a modified thrombin cleavage sites were amplified and cloned into pFastBacl, 
      the donor plasmid of Bac-to-Bac baculovirus expression systems, downstream from 
      the polyhedrin promoter (pPolh), resulting in the GST fusion transposition 
      plasmid pFGST. After transposition and transfection, SDS-PAGE analysis showed 
      that the developed GST fusion expression system can highly express GST. The 
      signal sequences of the major structural protein gene ORF5 of porcine 
      reproductive and respiratory syndrome virus (PRRSV) strain YA was removed by PCR 
      and the truncated ORF5 gene was inserted into pFGST and fused with GST to 
      generate the transposition plasmid expressing GST-ORF5 fusion protein. The 
      recombinant baculovirus rvBacGST53 was obtained by transposition and 
      transfection. GST-ORF5 fusion protein was identified by SDS-PAGE and Western 
      blot. The fusion protein is 45kD and is specific to the monoclonal antibody 
      against the E protein of PRRSV. The fusion protein was directly injected into 
      mice and the anti-sera can react with the PRRSV-infected MARC-145 cells by 
      indirect immunofluorescent assay (IFA), indicating that the fusion protein have 
      better immunogenicity.
FAU - Fang, Liurong
AU  - Fang L
AD  - Laboratory of Animal Virology, College of Animal Science and Veterinary Medicine, 
      Huazhong Agricultural University, Wuhan 430070, China.
FAU - Xiao, Shaobo
AU  - Xiao S
FAU - Niu, Chuanshuang
AU  - Niu C
FAU - Zhang, Hui
AU  - Zhang H
FAU - Chen, Huanchun
AU  - Chen H
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Wei Sheng Wu Xue Bao
JT  - Wei sheng wu xue bao = Acta microbiologica Sinica
JID - 21610860R
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Animals
MH  - Fluorescent Antibody Technique, Indirect
MH  - Glutathione Transferase/*genetics
MH  - Porcine respiratory and reproductive syndrome virus/*genetics
MH  - Recombinant Fusion Proteins/*biosynthesis
MH  - Spodoptera
MH  - Viral Envelope Proteins
MH  - Viral Proteins/*genetics
EDAT- 2005/11/10 09:00
MHDA- 2009/04/15 09:00
CRDT- 2005/11/10 09:00
PHST- 2005/11/10 09:00 [pubmed]
PHST- 2009/04/15 09:00 [medline]
PHST- 2005/11/10 09:00 [entrez]
PST - ppublish
SO  - Wei Sheng Wu Xue Bao. 2003 Feb;43(1):8-14.