PMID- 16277010
OWN - NLM
STAT- MEDLINE
DCOM- 20051206
LR  - 20131121
IS  - 0258-851X (Print)
IS  - 0258-851X (Linking)
VI  - 19
IP  - 6
DP  - 2005 Nov-Dec
TI  - Hidden chromosome 8 abnormalities detected by FISH in adult primary 
      myelodysplastic syndromes.
PG  - 979-81
AB  - Acquired clonal chromosomal abnormalities are found in about 30-50% of primary 
      myelodysplastic syndromes (MDS). These abnormalities are predominantly 
      characterized by total/partial chromosomal losses or gains and rarely by balanced 
      structural aberrations. Trisomy 8 represents the most common chromosomal gain. In 
      the present study, the numerical aberration of chromosome 8 was evaluated by the 
      fluorescence in situ hybridization (FISH) technique in MDS, and the results 
      compared with those of conventional cytogenetics. Thirty adult patients with 
      primary MDS, 17 with a normal karyotype and 13 with several chromosomal 
      abnormalities except chromosome 8, were included in this study. On comparing the 
      results of FISH and conventional cytogenetics, a superiority of FISH over the 
      karyotype was detected in 3 cases. In one of them, further cytogenetic analysis 
      confirmed the FISH results. Nevertheless, the FISH technique has limitations, 
      detecting only abnormalities specific for the target FISH probe used In clinical 
      practice, conventional cytogenetics continues to be the basic technique for MDS 
      patient evaluation. However, a large number of metaphases, even those of poor 
      quality, must be analyzed in each case. The FISH technique could be considered to 
      be complementary to achieve a more accurate analysis.
FAU - Panani, Anna D
AU  - Panani AD
AD  - Critical Care Department, Medical School of Athens University, Research Unit, 
      Evangelismos Hospital, Athens, Greece. apanani@med.uoa.gr
FAU - Pappa, Vasiliki
AU  - Pappa V
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Greece
TA  - In Vivo
JT  - In vivo (Athens, Greece)
JID - 8806809
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Indoles)
RN  - 47165-04-8 (DAPI)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Adult
MH  - Cell Nucleus/metabolism
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 8
MH  - Cytogenetic Analysis
MH  - Fluorescein-5-isothiocyanate
MH  - Fluorescent Dyes
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Indoles
MH  - Karyotyping
MH  - Metaphase
MH  - Microscopy, Fluorescence
MH  - Myelodysplastic Syndromes/*diagnosis/*genetics
MH  - Prognosis
MH  - Trisomy
EDAT- 2005/11/10 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/11/10 09:00
PHST- 2005/11/10 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/11/10 09:00 [entrez]
PST - ppublish
SO  - In Vivo. 2005 Nov-Dec;19(6):979-81.