PMID- 16277839
OWN - NLM
STAT- MEDLINE
DCOM- 20080905
LR  - 20161018
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 13
IP  - 5
DP  - 2005 Oct
TI  - [Mechanism of apoptosis induced by indole-3-acetic acids combined with 
      horseradish peroxidase in leukemia cell line K562].
PG  - 769-73
AB  - To investigate the possible mechanism of apoptosis induced by indole-3-acetic 
      acid (IAA) combined with horseradish peroxidase in leukemia cell line K562, cell 
      proliferation and apoptosis of K562 cell were examined by MTT assay and terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), 
      respectively; the activity of superoxide dismutase (SOD) and the quantitative 
      change of MDA were measured by biochemical method; changes of free radical were 
      determined by 2, 7-dichlorofluorescin diacetate (DCFH-DA) probe with confocal 
      microscopy. The results showed that of MTT assay and TUNEL indicated that IAA/HRP 
      could significantly inhibit cell proliferation (P < 0.05) and induce apoptosis of 
      K562 cell (P < 0.01), at the same time a positive correlation was found between 
      apoptosis rate and IAA concentration (r = 0.971, P < 0.01). The activity of SOD 
      and the quantitative of MDA increased, accompanied with a rise in IAA 
      concentration. Results detected by DCFH-DA probe indicated that the fluorescence 
      intensity of intracellular free radical increased, as compared with control, and 
      a positive correlation was found. It is concluded that IAA/HRP can inhibit 
      proliferation of K562 cells and induce K562 cell apoptosis, its mechanism may be 
      related with the increase of intracellular free radical due to the effects of 
      IAA/HRP.
FAU - Yang, Ling
AU  - Yang L
AD  - Department of Biology and Medical Genetics, Medical College of Xi'an Jiaotong 
      University, Xi'an 710061, China.
FAU - Song, Tu-Sheng
AU  - Song TS
FAU - Huang, Chen
AU  - Huang C
FAU - Liu, Li-Ying
AU  - Liu LY
FAU - Luo, Yu
AU  - Luo Y
FAU - Ni, Lei
AU  - Ni L
FAU - Si, Lu-Sheng
AU  - Si LS
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
RN  - 0 (Indoleacetic Acids)
RN  - 0 (Reactive Oxygen Species)
RN  - 6U1S09C61L (indoleacetic acid)
RN  - EC 1.11.1.- (Horseradish Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Drug Synergism
MH  - Horseradish Peroxidase/*pharmacology
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Indoleacetic Acids/*pharmacology
MH  - K562 Cells
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/metabolism/pathology
MH  - Microscopy, Confocal
MH  - Reactive Oxygen Species/metabolism
MH  - Superoxide Dismutase/metabolism
MH  - Time Factors
EDAT- 2005/11/10 09:00
MHDA- 2008/09/06 09:00
CRDT- 2005/11/10 09:00
PHST- 2005/11/10 09:00 [pubmed]
PHST- 2008/09/06 09:00 [medline]
PHST- 2005/11/10 09:00 [entrez]
AID - 1009-2137(2005)05-0769-05 [pii]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2005 Oct;13(5):769-73.