PMID- 16279947
OWN - NLM
STAT- MEDLINE
DCOM- 20051230
LR  - 20191210
IS  - 1742-464X (Print)
IS  - 1742-464X (Linking)
VI  - 272
IP  - 22
DP  - 2005 Nov
TI  - Molecular identification of adrenal inner zone antigen as a heme-binding protein.
PG  - 5832-43
AB  - The adrenal inner zone antigen (IZA), which reacts specifically with a monoclonal 
      antibody raised against the fasciculata and reticularis zones of the rat adrenal, 
      was previously found to be identical with a protein variously named 25-Dx and 
      membrane-associated progesterone receptor. IZA was purified as a glutathione 
      S-transferase-fused or His(6)-fused protein, and its molecular properties were 
      studied. The UV-visible absorption and EPR spectra of the purified protein showed 
      that IZA bound a heme chromophore in high-spin type. Analysis of the heme 
      indicated that it is of the b type. Site-directed mutagenesis studies were 
      performed to identify the amino-acid residues that bind the heme to the protein. 
      The results suggest that two Tyr residues, Tyr107 and Tyr113, and a peptide 
      stretch, D99-K102, were important for anchoring the heme into a hydrophobic 
      pocket. The effect of IZA on the steroid 21-hydroxylation reaction was 
      investigated in COS-7 cell expression systems. The results suggest that the 
      coexistence of IZA with CYP21 enhances 21-hydroxylase activity.
FAU - Min, Li
AU  - Min L
AD  - Department of Molecular Physiological Chemistry, Graduate School of Medicine, 
      Osaka University, Japan.
FAU - Strushkevich, Natallia V
AU  - Strushkevich NV
FAU - Harnastai, Ivan N
AU  - Harnastai IN
FAU - Iwamoto, Hiroko
AU  - Iwamoto H
FAU - Gilep, Andrei A
AU  - Gilep AA
FAU - Takemori, Hiroshi
AU  - Takemori H
FAU - Usanov, Sergey A
AU  - Usanov SA
FAU - Nonaka, Yasuki
AU  - Nonaka Y
FAU - Hori, Hiroshi
AU  - Hori H
FAU - Vinson, Gavin P
AU  - Vinson GP
FAU - Okamoto, Mitsuhiro
AU  - Okamoto M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS J
JT  - The FEBS journal
JID - 101229646
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens)
RN  - 0 (Carrier Proteins)
RN  - 0 (Heme-Binding Proteins)
RN  - 0 (Hemeproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Pgrmc1 protein, rat)
RN  - 0 (Receptors, Progesterone)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 4QD397987E (Histidine)
RN  - EC 1.13.12.- (Luciferases)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Adrenal Cortex/cytology/*metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Monoclonal/metabolism
MH  - Antigens/*metabolism
MH  - COS Cells
MH  - Carrier Proteins/analysis/chemistry/genetics/*metabolism
MH  - Chlorocebus aethiops
MH  - Cold Temperature
MH  - Electron Spin Resonance Spectroscopy
MH  - Escherichia coli/genetics
MH  - Genes, Reporter
MH  - Glutathione Transferase/metabolism
MH  - HeLa Cells
MH  - Heme-Binding Proteins
MH  - Hemeproteins/analysis/chemistry/*metabolism
MH  - Histidine/chemistry
MH  - Humans
MH  - Luciferases/metabolism
MH  - Membrane Proteins
MH  - Microscopy, Fluorescence
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - Protein Binding
MH  - Protein Structure, Secondary
MH  - Protein Structure, Tertiary
MH  - Rats
MH  - Receptors, Progesterone/chemistry/genetics/*metabolism
MH  - Recombinant Fusion Proteins/chemistry/isolation & purification/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Spectrophotometry, Ultraviolet
MH  - Zona Fasciculata/cytology/metabolism
MH  - Zona Reticularis/cytology/metabolism
EDAT- 2005/11/11 09:00
MHDA- 2005/12/31 09:00
CRDT- 2005/11/11 09:00
PHST- 2005/11/11 09:00 [pubmed]
PHST- 2005/12/31 09:00 [medline]
PHST- 2005/11/11 09:00 [entrez]
AID - EJB4977 [pii]
AID - 10.1111/j.1742-4658.2005.04977.x [doi]
PST - ppublish
SO  - FEBS J. 2005 Nov;272(22):5832-43. doi: 10.1111/j.1742-4658.2005.04977.x.