PMID- 16280633
OWN - NLM
STAT- MEDLINE
DCOM- 20060224
LR  - 20190621
IS  - 0378-5866 (Print)
IS  - 0378-5866 (Linking)
VI  - 27
IP  - 6
DP  - 2005
TI  - Serotonin transporter function is an early step in left-right patterning in chick 
      and frog embryos.
PG  - 349-63
AB  - The neurotransmitter serotonin has been shown to regulate a number of embryonic 
      patterning events in addition to its crucial role in the nervous system. Here, we 
      examine the role of two serotonin transporters, the plasma membrane serotonin 
      transporter (SERT) and the vesicular monoamine transporter (VMAT), in embryonic 
      left-right asymmetry. Pharmacological or genetic inhibitors of either SERT or 
      VMAT specifically randomized the laterality of the heart and viscera in Xenopus 
      embryos. This effect takes place during cleavage stages, and is upstream of the 
      left-sided gene XNR-1. Targeted microinjection of an SERT-dominant negative 
      construct confirmed the necessity for SERT function in embryonic laterality and 
      revealed that the descendants of the right ventral blastomere are the most 
      dependent upon SERT signaling in left-right patterning. Moreover, the importance 
      of SERT and VMAT in laterality is conserved in chick embryos, being upstream of 
      the early left-sided gene Shh. Endogenous transcripts of SERT and VMAT are 
      expressed from the initiation of the primitive streak in chick and are 
      asymmetrically expressed in Hensen's node. Taken together our data characterize 
      two new right-sided markers in chick gastrulation, identify a novel, early 
      component of the left-right pathway in two vertebrate species, and reveal a new 
      biological role for serotonin transport.
CI  - Copyright (c) 2005 S. Karger AG, Basel.
FAU - Fukumoto, Takahiro
AU  - Fukumoto T
AD  - Cytokine Biology Department, The Forsyth Institute, Harvard Medical School, 
      Boston, Mass. 02115, USA.
FAU - Blakely, Randy
AU  - Blakely R
FAU - Levin, Michael
AU  - Levin M
LA  - eng
GR  - CO6RR11244/CO/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Switzerland
TA  - Dev Neurosci
JT  - Developmental neuroscience
JID - 7809375
RN  - 0 (Hedgehog Proteins)
RN  - 0 (Serotonin Plasma Membrane Transport Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Vesicular Monoamine Transport Proteins)
RN  - 0 (Xenopus Proteins)
RN  - 0 (nodal1 protein, Xenopus)
RN  - 333DO1RDJY (Serotonin)
SB  - IM
MH  - Animals
MH  - Body Patterning/*genetics
MH  - Chick Embryo
MH  - Chickens
MH  - Embryo, Nonmammalian/cytology/embryology/metabolism
MH  - Embryonic Development/*genetics
MH  - Gastrula/cytology/metabolism
MH  - Gene Expression Regulation, Developmental/*genetics
MH  - Hedgehog Proteins
MH  - Organogenesis/genetics
MH  - Serotonin/metabolism
MH  - Serotonin Plasma Membrane Transport Proteins/drug effects/*genetics
MH  - Trans-Activators/genetics
MH  - Transcriptional Activation/genetics
MH  - Transforming Growth Factor beta/genetics
MH  - Vesicular Monoamine Transport Proteins/antagonists & inhibitors/*genetics
MH  - Xenopus Proteins
MH  - Xenopus laevis
EDAT- 2005/11/11 09:00
MHDA- 2006/02/25 09:00
CRDT- 2005/11/11 09:00
PHST- 2005/04/06 00:00 [received]
PHST- 2005/04/22 00:00 [accepted]
PHST- 2005/11/11 09:00 [pubmed]
PHST- 2006/02/25 09:00 [medline]
PHST- 2005/11/11 09:00 [entrez]
AID - 88451 [pii]
AID - 10.1159/000088451 [doi]
PST - ppublish
SO  - Dev Neurosci. 2005;27(6):349-63. doi: 10.1159/000088451.