PMID- 16282532
OWN - NLM
STAT- MEDLINE
DCOM- 20060511
LR  - 20150813
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 79
IP  - 2
DP  - 2006 Feb
TI  - Mast cells, which interact with Escherichia coli, up-regulate genes associated 
      with innate immunity and become less responsive to Fc(epsilon)RI-mediated 
      activation.
PG  - 339-50
AB  - Mast cells, which are associated with T helper cell type 2-dependent 
      inflammation, have now been implicated in the innate immune response. To further 
      characterize how mast cells are programmed to respond to infectious organisms, we 
      used expression profiling using DNA microarray analysis of gene expression by 
      human mast cells (huMC) during ingestion of Escherichia coli and examined 
      immunoglobulin E (IgE)-mediated degranulation. Analysis of data revealed that 
      specific groups of genes were modulated, including genes encoding transcription 
      factors, cell signaling molecules, cell cycle regulators, enzymes, cytokines, 
      novel chemokines of the CC family, adhesion molecules, and costimulatory 
      molecules. Enzyme-linked immunosorbent assay analysis confirmed the production of 
      tumor necrosis factor and the chemokines CC chemokine ligand (CCL)-1/I-309, 
      CCL-19/macrophage-inflammatory protein-3beta (MIP-3beta), and CCL-18/MIP-4; flow 
      cytometry confirmed the up-regulation of carcinoembryonic antigen-related cell 
      adhesion molecule 1, the integrin CD49d, and CD80. Coincubation with E. coli 
      down-regulated Fc receptor for IgE I (FcepsilonRI) expression and 
      FcepsilonRI-mediated huMC degranulation. These data are consistent with the 
      concept that bacterial exposure directs mast cell responses toward innate 
      immunity and away from IgE-mediated effects.
FAU - Kulka, Marianna
AU  - Kulka M
AD  - Allergy-Immunology Division, Northwestern University Feinberg School of Medicine, 
      Chicago, IL, USA.
FAU - Fukuishi, Nobuyuki
AU  - Fukuishi N
FAU - Rottem, Menachem
AU  - Rottem M
FAU - Mekori, Yoseph A
AU  - Mekori YA
FAU - Metcalfe, Dean D
AU  - Metcalfe DD
LA  - eng
GR  - Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
DEP - 20051110
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Chemokines)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, IgE)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Cell Adhesion Molecules/immunology
MH  - Cells, Cultured
MH  - Chemokines/genetics/immunology
MH  - Escherichia coli/*immunology
MH  - Gene Expression Regulation/immunology
MH  - Humans
MH  - *Immunity, Innate
MH  - Immunoglobulin E/immunology
MH  - Mast Cells/*immunology
MH  - Oligonucleotide Array Sequence Analysis/methods
MH  - RNA, Messenger/genetics
MH  - Receptors, IgE/genetics/*immunology
MH  - Tumor Necrosis Factor-alpha/genetics/immunology
MH  - Up-Regulation/immunology
EDAT- 2005/11/12 09:00
MHDA- 2006/05/12 09:00
CRDT- 2005/11/12 09:00
PHST- 2005/11/12 09:00 [pubmed]
PHST- 2006/05/12 09:00 [medline]
PHST- 2005/11/12 09:00 [entrez]
AID - jlb.1004600 [pii]
AID - 10.1189/jlb.1004600 [doi]
PST - ppublish
SO  - J Leukoc Biol. 2006 Feb;79(2):339-50. doi: 10.1189/jlb.1004600. Epub 2005 Nov 10.