PMID- 16283718
OWN - NLM
STAT- MEDLINE
DCOM- 20060207
LR  - 20131121
IS  - 1525-7797 (Print)
IS  - 1525-7797 (Linking)
VI  - 6
IP  - 6
DP  - 2005 Nov-Dec
TI  - Synthesis, characterization, and evaluation as transfection reagents of 
      double-hydrophilic star copolymers: effect of star architecture.
PG  - 2990-7
AB  - Five star polymers of the ionizable hydrophilic 2-(dimethylamino)ethyl 
      methacrylate (DMAEMA) and the nonionic hydrophilic methoxy hexa(ethylene glycol) 
      methacrylate (HEGMA) were prepared by group transfer polymerization (GTP) using 
      ethylene glycol dimethacrylate (EGDMA) as coupling agent. In particular, four 
      isomeric star copolymers, one heteroarm, two star block and one statistical star, 
      with 90% mol DMAEMA and 10% mol HEGMA, plus one star homopolymer of DMAEMA with 
      degrees of polymerization of the arms equal to 20 were synthesized. The polymers 
      were characterized in terms of their molar masses (MMs) and compositions using 
      gel permeation chromatography (GPC) and proton nuclear magnetic resonance (1H 
      NMR) spectroscopy, respectively. The hydrodynamic diameters in water indicated 
      some aggregation for all the star polymers except for the statistical copolymer 
      star, while the pK values of the DMAEMA units were around 7 for all star 
      polymers. All the star polymers were evaluated for their ability to transfect 
      human cervical HeLa cancer cells with the modified plasmid pRLSV40 bearing the 
      enhanced green fluorescent protein (EGFP) as the reporter gene. All four star 
      copolymers showed decreased toxicity compared to that of the DMAEMA star 
      homopolymer for the same amounts of star polymer tested. The star block copolymer 
      with outer DMAEMA blocks exhibited the highest overall transfection efficiency, 
      11%, compared to that of all the star polymers examined in this study. This 
      efficiency was the same as that of the commercially available transfection 
      reagent SuperFect.
FAU - Georgiou, Theoni K
AU  - Georgiou TK
AD  - Department of Chemistry, University of Cyprus, P.O. Box 20537, 1678 Nicosia, 
      Cyprus, Department of Materials Science and Technology, University of Crete, P.O. 
      Box 2208, 710 03 Heraklion, Crete, Greece.
FAU - Vamvakaki, Maria
AU  - Vamvakaki M
FAU - Phylactou, Leonidas A
AU  - Phylactou LA
FAU - Patrickios, Costas S
AU  - Patrickios CS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biomacromolecules
JT  - Biomacromolecules
JID - 100892849
RN  - 0 (Biocompatible Materials)
RN  - 0 (Ethylene Glycols)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Methacrylates)
RN  - 0 (Polymers)
RN  - 0 (hydrophilic methoxy hexa(ethylene glycol) methacrylate)
RN  - 059QF0KO0R (Water)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 7BK5G69305 (ethylene dimethacrylate)
RN  - 9007-49-2 (DNA)
RN  - O0V97PV2G1 (2-(dimethylamino)ethyl methacrylate)
SB  - IM
MH  - Biochemistry/methods
MH  - Biocompatible Materials/*chemistry
MH  - Chromatography, Gel
MH  - DNA/chemistry
MH  - Dose-Response Relationship, Drug
MH  - Ethylene Glycols/*chemistry
MH  - Genes, Reporter
MH  - Green Fluorescent Proteins/metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Light
MH  - Macromolecular Substances/chemistry
MH  - Magnetic Resonance Spectroscopy
MH  - Methacrylates/*chemistry
MH  - Models, Chemical
MH  - Nephelometry and Turbidimetry
MH  - Plasmids/metabolism
MH  - Polymers/*chemistry
MH  - Scattering, Radiation
MH  - Transfection
MH  - Water/chemistry
EDAT- 2005/11/15 09:00
MHDA- 2006/02/08 09:00
CRDT- 2005/11/15 09:00
PHST- 2005/11/15 09:00 [pubmed]
PHST- 2006/02/08 09:00 [medline]
PHST- 2005/11/15 09:00 [entrez]
AID - 10.1021/bm050307w [doi]
PST - ppublish
SO  - Biomacromolecules. 2005 Nov-Dec;6(6):2990-7. doi: 10.1021/bm050307w.