PMID- 16284003
OWN - NLM
STAT- MEDLINE
DCOM- 20060127
LR  - 20161124
IS  - 1073-9688 (Print)
IS  - 1073-9688 (Linking)
VI  - 12
IP  - 8
DP  - 2005 Dec
TI  - Protective effects of ischemic preconditioning on the intestinal mucosal 
      microcirculation following ischemia-reperfusion of the intestine.
PG  - 615-25
AB  - OBJECTIVE: The small bowel villi are extremely sensitive to ischemia-reperfusion 
      (IR) injury and a range of microcirculatory disturbances contribute to structural 
      and functional changes. The aim of this study was to determine the protective 
      effects of ischemic preconditioning (IPC) of the intestine on the mucosal villous 
      microcirculation during IR injury of the intestine and whether heme oxygenase 
      (HO) is involved in the protection. METHODS: Rats were allocated into 4 groups: 
      (1) sham, (2) IR consisting of 30 min of ischemia followed by 2 h of reperfusion, 
      (3) IPC, as in IR group, but preceded by 10 min of ischemia and 10 min of 
      reperfusion, and (4) with administration of zinc protoporphyrin, an HO inhibitor 
      before IPC and IR. The mucosa of an exteriorized segment of ileum was visualized. 
      Mucosal perfusion index (MPI), red blood cell (RBC) velocity and 
      leukocyte-endothelial interactions during reperfusion were assessed continuously 
      using in vivo fluorescence microscopy. HO activity in the ileum was assessed at 
      the end of the reperfusion period. RESULTS: IPC improved the MPI by 26% and the 
      RBC velocity by 29% on comparison to IR. IR led to a 52% increase in 
      leukocyte-endothelial interactions on comparison to IPC. The administration of 
      zinc protoporphyrin reversed the beneficial effects of IPC. There was a two fold 
      increase of HO activity in IPC compared to IR, whereas zinc protoporphyrin 
      significantly reduced the HO activity. CONCLUSIONS: IPC conferred a protective 
      effect on the villous microcirculation possibly via HO and might prove to be an 
      effective strategy for the amelioration of IR injury.
FAU - Mallick, Ismail H
AU  - Mallick IH
AD  - GI & Hepatobiliary Research Lab, Academic Division of Surgical and Interventional 
      Sciences, University College London, London, United Kingdom.
FAU - Yang, Wenxuan
AU  - Yang W
FAU - Winslet, Marc C
AU  - Winslet MC
FAU - Seifalian, Alexander M
AU  - Seifalian AM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Microcirculation
JT  - Microcirculation (New York, N.Y. : 1994)
JID - 9434935
RN  - EC 1.14.14.18 (Heme Oxygenase (Decyclizing))
SB  - IM
MH  - Animals
MH  - Heme Oxygenase (Decyclizing)/*metabolism
MH  - Ileum/blood supply/*enzymology/physiology
MH  - Intestinal Mucosa/blood supply/*enzymology/pathology
MH  - *Ischemic Preconditioning
MH  - Male
MH  - Microcirculation/enzymology/pathology
MH  - Microvilli/enzymology/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reperfusion Injury/*enzymology/pathology
EDAT- 2005/11/15 09:00
MHDA- 2006/01/28 09:00
CRDT- 2005/11/15 09:00
PHST- 2005/11/15 09:00 [pubmed]
PHST- 2006/01/28 09:00 [medline]
PHST- 2005/11/15 09:00 [entrez]
AID - P1H1106H1531R616 [pii]
AID - 10.1080/10739680500301631 [doi]
PST - ppublish
SO  - Microcirculation. 2005 Dec;12(8):615-25. doi: 10.1080/10739680500301631.