PMID- 16284217
OWN - NLM
STAT- MEDLINE
DCOM- 20060214
LR  - 20190108
IS  - 0040-6376 (Print)
IS  - 1468-3296 (Electronic)
IS  - 0040-6376 (Linking)
VI  - 61
IP  - 2
DP  - 2006 Feb
TI  - Neutrophilic inflammation and IL-8 levels in induced sputum of 
      alpha-1-antitrypsin PiMZ subjects.
PG  - 129-33
AB  - BACKGROUND: Severe alpha-1-antitrypsin deficiency (AATD), due to homozygosity for 
      the protease inhibitor (Pi) Z allele, is a genetic risk factor for chronic 
      obstructive pulmonary disease (COPD). In a previous study the sputum of severe 
      AATD subjects with airflow obstruction showed a pattern of cellular inflammation 
      similar to COPD patients. It is uncertain whether heterozygotes for the Z allele 
      or intermediate deficiency (PiMZ) have an increased risk of developing COPD. 
      METHODS: Sputum cell counts and the supernatant level of the neutrophil 
      chemoattractant interleukin (IL)-8 were investigated by sputum induction in 10 
      non-smoker asymptomatic PiMZ subjects with normal pulmonary function, 10 patients 
      with stable COPD, and 10 age matched normal subjects. Data are expressed as mean 
      (SD). RESULTS: The mean (SD) number of neutrophils was significantly higher 
      (p<0.01) in the sputum of PiMZ subjects (84.5 (22.2) x10(4)/ml) and patients with 
      COPD (126.9 (18.8) x10(4)/ml) than in matched normal subjects (55.0 (8.7) 
      x10(4)/ml). IL-8 levels were increased in PiMZ subjects (828.5 (490.6) ng/ml; 
      median 1003.0 ng/ml; range 1260-100 ng/ml) and in COPD patients (882.5 (524.3) 
      ng/ml; median 934.9 ng/ml; range 1506-258 mg/ml) compared with normal subjects 
      (3.5 (0.5) ng/ml; median 3.5 ng/ml; range 4.5-2.5 ng/ml). There was a significant 
      positive correlation between IL-8 supernatant concentration and neutrophil count 
      in PiMZ subjects (p = 0.036; r = 0.66). An inverse correlation was observed 
      between the percentage of neutrophils and forced expiratory volume in 1 second (% 
      predicted) in patients with COPD (p = 0.04; r = -0.43). CONCLUSIONS: These 
      findings indicate that PiMZ subjects without airflow obstruction may have an IL-8 
      related neutrophilic inflammation in the airways, similar to stable COPD 
      patients, suggesting an increased risk of developing pulmonary changes.
FAU - Malerba, M
AU  - Malerba M
AD  - Department of Internal Medicine, University of Brescia, 1st Divisione di 
      Medicina, Spedali Civili, Piazza Spedali Civili 1, 25100 Brescia, Italy. 
      malerba@master.cci.unibs.it
FAU - Ricciardolo, F
AU  - Ricciardolo F
FAU - Radaeli, A
AU  - Radaeli A
FAU - Torregiani, C
AU  - Torregiani C
FAU - Ceriani, L
AU  - Ceriani L
FAU - Mori, E
AU  - Mori E
FAU - Bontempelli, M
AU  - Bontempelli M
FAU - Tantucci, C
AU  - Tantucci C
FAU - Grassi, V
AU  - Grassi V
LA  - eng
PT  - Journal Article
DEP - 20051111
PL  - England
TA  - Thorax
JT  - Thorax
JID - 0417353
RN  - 0 (Interleukin-8)
RN  - 1HGW4DR56D (Leukotriene B4)
RN  - 7U1EE4V452 (Carbon Monoxide)
SB  - IM
MH  - Aged
MH  - Bronchitis/*metabolism/pathology
MH  - Carbon Monoxide/metabolism
MH  - Case-Control Studies
MH  - Female
MH  - Forced Expiratory Volume/physiology
MH  - Humans
MH  - Interleukin-8/*metabolism
MH  - Leukotriene B4/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Neutrophils/pathology
MH  - Sputum/cytology
MH  - Vital Capacity/physiology
MH  - alpha 1-Antitrypsin Deficiency/*metabolism/pathology/physiopathology
PMC - PMC2104580
COIS- Conflict of interest: none declared.
EDAT- 2005/11/15 09:00
MHDA- 2006/02/16 09:00
PMCR- 2009/02/01
CRDT- 2005/11/15 09:00
PHST- 2005/11/15 09:00 [pubmed]
PHST- 2006/02/16 09:00 [medline]
PHST- 2005/11/15 09:00 [entrez]
PHST- 2009/02/01 00:00 [pmc-release]
AID - thx.2005.043471 [pii]
AID - tx43471 [pii]
AID - 10.1136/thx.2005.043471 [doi]
PST - ppublish
SO  - Thorax. 2006 Feb;61(2):129-33. doi: 10.1136/thx.2005.043471. Epub 2005 Nov 11.