PMID- 16284749
OWN - NLM
STAT- MEDLINE
DCOM- 20060314
LR  - 20121115
IS  - 0302-8933 (Print)
IS  - 0302-8933 (Linking)
VI  - 184
IP  - 5
DP  - 2006 Jan
TI  - Isolation and characterization of a gene cluster for dibenzofuran degradation in 
      a new dibenzofuran-utilizing bacterium, Paenibacillus sp. strain YK5.
PG  - 305-15
AB  - Spore-forming bacterial strains capable of utilizing dibenzofuran (DF) as a sole 
      source of carbon and energy were isolated. Characteristics of the isolates 
      justified their classification into the genus Paenibacillus, and their closest 
      relative was P. naphthalenovorans. Degenerate primers for aromatic hydrocarbon 
      dioxygenase alpha subunit (AhDOa) genes and genomic DNA of the strain YK5 were 
      used for gene isolation. The nucleotide sequences of clones of the PCR products 
      revealed that the strain YK5 carries at least five different AhDOa genes. 
      Northern hybridization analysis showed that one of the AhDOa genes was 
      transcribed under DF-containing culture conditions. A gene cluster encoding the 
      AhDOa was isolated. The genes predicted to encode extradiol dioxygenase (dbfB) 
      and hydrolase (dbfC) were found to be an upstream of genes encoding the alpha and 
      beta subunit of the AhDO (dbfA1 and dbfA2, respectively); the latter two gene 
      products showed 60 and 53% identity to the amino acid sequences of DbfA1 and 
      DbfA2 of Terrabacter sp. DBF63, respectively. Two Paenibacillus validus JCM 9077 
      strains transformed with the dbf gene clusters acquired the ability to convert DF 
      to 2,2',3-trihydroxybiphenyl (THBP) and salicylic acid (SAL). These results 
      suggest that the enzymes encoded by the gene cluster isolated in this study are 
      involved in DF metabolism in YK5.
FAU - Iida, Toshiya
AU  - Iida T
AD  - Environmental Molecular Biology Laboratory, RIKEN, Wako, Saitama 351-0198, Japan. 
      tiida@postman.riken.go.jp
FAU - Nakamura, Kaoru
AU  - Nakamura K
FAU - Izumi, Atsushi
AU  - Izumi A
FAU - Mukouzaka, Yuki
AU  - Mukouzaka Y
FAU - Kudo, Toshiaki
AU  - Kudo T
LA  - eng
SI  - GENBANK/AB201838
SI  - GENBANK/AB201839
SI  - GENBANK/AB201840
SI  - GENBANK/AB201841
SI  - GENBANK/AB201842
SI  - GENBANK/AB201843
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051112
PL  - Germany
TA  - Arch Microbiol
JT  - Archives of microbiology
JID - 0410427
RN  - 0 (Benzofurans)
RN  - 0 (Hydrocarbons, Aromatic)
RN  - 8U54U639VI (dibenzofuran)
RN  - EC 1.13.- (Oxygenases)
RN  - EC 1.13.11.- (Dioxygenases)
RN  - EC 1.14.99.- (extradiol dioxygenase)
RN  - EC 3.- (Hydrolases)
SB  - IM
MH  - Base Composition
MH  - Benzofurans/*metabolism
MH  - Cloning, Molecular
MH  - Dioxygenases/genetics/metabolism
MH  - Gram-Positive Bacteria/classification/*enzymology/genetics/physiology
MH  - Hydrocarbons, Aromatic/metabolism
MH  - Hydrolases/genetics/metabolism
MH  - Molecular Sequence Data
MH  - *Multigene Family
MH  - Oxygenases/genetics/metabolism
MH  - Sequence Analysis, DNA
MH  - Spores, Bacterial/physiology
EDAT- 2005/11/15 09:00
MHDA- 2006/03/15 09:00
CRDT- 2005/11/15 09:00
PHST- 2005/04/13 00:00 [received]
PHST- 2005/09/19 00:00 [accepted]
PHST- 2005/08/15 00:00 [revised]
PHST- 2005/11/15 09:00 [pubmed]
PHST- 2006/03/15 09:00 [medline]
PHST- 2005/11/15 09:00 [entrez]
AID - 10.1007/s00203-005-0045-9 [doi]
PST - ppublish
SO  - Arch Microbiol. 2006 Jan;184(5):305-15. doi: 10.1007/s00203-005-0045-9. Epub 2005 
      Nov 12.