PMID- 16287102 OWN - NLM STAT- MEDLINE DCOM- 20060630 LR - 20081121 IS - 0275-2565 (Print) IS - 0275-2565 (Linking) VI - 67 IP - 3 DP - 2005 Nov TI - Multiplexed microsatellites for rapid identification and characterization of individuals and populations of Cercopithecidae. PG - 385-91 AB - Cross-amplification of 15 human microsatellites was performed successfully in cynomolgus (Macaca fascicularis) and rhesus (M. mulatta) macaques and 11 other Cercopithecidae species of biomedical and conservation relevance. To allow for quick, efficient, and high-throughput genotyping to assess intra- and interspecific genetic variation, we performed three multiplex sets, each comprised of five markers from different parts of the genome (i.e., autosomes, the MHC region, and the X-chromosome). These multiplex sets are likely to reveal allelic divergence between taxa, which could be used for their discrimination. Population studies on three regional populations of M. fascicularis and one of M. mulatta revealed that most of the loci, with the exception of one monomorphic locus, displayed polymorphisms (the expected heterozygosities were 0.48-0.91 for M. fascicularis, and 0.61-0.93 for M. mulatta), which makes them useful for population genetics. For the multiplex set M1, including the nonlinked autosomal markers, low probabilities of identity were observed: P(ID) values ranged from 8 x 10(-7) to 3 x 10(-5). This multiplex set is reliable for forensic applications, such as individual identification, parentage testing, and kinship analysis, in wild and captive populations. FAU - Bonhomme, M AU - Bonhomme M AD - Universite Paul Sabatier, Laboratoire Evolution et Diversite Biologique EDB, Toulouse, France. FAU - Blancher, A AU - Blancher A FAU - Crouau-Roy, B AU - Crouau-Roy B LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Am J Primatol JT - American journal of primatology JID - 8108949 RN - 0 (Genetic Markers) SB - IM MH - Animal Identification Systems/*methods MH - Animals MH - Cercopithecidae/*genetics MH - Genetic Markers/genetics MH - Genetic Variation/genetics MH - Genotype MH - Microsatellite Repeats/*genetics MH - Species Specificity MH - Time Factors EDAT- 2005/11/16 09:00 MHDA- 2006/07/01 09:00 CRDT- 2005/11/16 09:00 PHST- 2005/11/16 09:00 [pubmed] PHST- 2006/07/01 09:00 [medline] PHST- 2005/11/16 09:00 [entrez] AID - 10.1002/ajp.20193 [doi] PST - ppublish SO - Am J Primatol. 2005 Nov;67(3):385-91. doi: 10.1002/ajp.20193.