PMID- 16289391
OWN - NLM
STAT- MEDLINE
DCOM- 20060824
LR  - 20061115
IS  - 0167-7012 (Print)
IS  - 0167-7012 (Linking)
VI  - 66
IP  - 1
DP  - 2006 Jul
TI  - Comparison of methods and animal models commonly used for investigation of fecal 
      microbiota: effects of time, host and gender.
PG  - 87-95
AB  - Denaturing gradient gel electrophoresis (DGGE), terminal restriction fragment 
      length polymorphism (T-RFLP) and plating on selective agars were used to study 
      variation in the fecal microbiota of rats over time as well as variation between 
      individuals. Investigated rats were either conventional and specific pathogen 
      free (SPF), or human flora associated (HFA). A higher variation (p<0.05) in fecal 
      microbiota over time was observed for HFA than for SPF animals. Analysis of DGGE 
      and T-RFLP profiles of fecal microbiota from SPF and HFA rats revealed that 
      variation over time was less significant than variation between individuals, and 
      that phylogenetic profiles clustered according to gender. These observations 
      should be taken into account when designing future research addressing changes in 
      fecal microbiota.
FAU - Bernbom, Nete
AU  - Bernbom N
AD  - Department of Microbiological Food Safety, Danish Institute for Food and 
      Veterinary Research, Morkhoj Bygade 19, DK-2860 Soborg, Denmark.
FAU - Norrung, Birgit
AU  - Norrung B
FAU - Saadbye, Peter
AU  - Saadbye P
FAU - Molbak, Lars
AU  - Molbak L
FAU - Vogensen, Finn K
AU  - Vogensen FK
FAU - Licht, Tine Rask
AU  - Licht TR
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051111
PL  - Netherlands
TA  - J Microbiol Methods
JT  - Journal of microbiological methods
JID - 8306883
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Colony Count, Microbial
MH  - DNA, Bacterial/chemistry/genetics
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Enterococcus/genetics/*isolation & purification
MH  - Feces/*microbiology
MH  - Female
MH  - Humans
MH  - Lactobacillus/genetics/*isolation & purification
MH  - Male
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Restriction Fragment Length
MH  - Principal Component Analysis
MH  - RNA, Ribosomal, 16S/chemistry/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Specific Pathogen-Free Organisms
MH  - Streptococcus/genetics/*isolation & purification
EDAT- 2005/11/18 09:00
MHDA- 2006/08/25 09:00
CRDT- 2005/11/18 09:00
PHST- 2005/08/09 00:00 [received]
PHST- 2005/10/14 00:00 [revised]
PHST- 2005/10/21 00:00 [accepted]
PHST- 2005/11/18 09:00 [pubmed]
PHST- 2006/08/25 09:00 [medline]
PHST- 2005/11/18 09:00 [entrez]
AID - S0167-7012(05)00333-7 [pii]
AID - 10.1016/j.mimet.2005.10.014 [doi]
PST - ppublish
SO  - J Microbiol Methods. 2006 Jul;66(1):87-95. doi: 10.1016/j.mimet.2005.10.014. Epub 
      2005 Nov 11.