PMID- 16290189
OWN - NLM
STAT- MEDLINE
DCOM- 20060503
LR  - 20181113
IS  - 1063-4584 (Print)
IS  - 1522-9653 (Electronic)
IS  - 1063-4584 (Linking)
VI  - 14
IP  - 3
DP  - 2006 Mar
TI  - Regulation of matrix metalloproteinase expression by dynamic tensile strain in 
      rat fibrochondrocytes.
PG  - 264-72
AB  - OBJECTIVE: We sought to determine the molecular basis for the anticatabolic 
      effects of mechanical signals on fibrocartilage cells by studying the expression 
      of a variety of matrix metalloproteinases (MMPs). Furthermore, we examined 
      whether the effects of biomechanical strain on MMP gene expression are sustained. 
      METHODS: Fibrochondrocytes from temporomandibular joint (TMJ) discs were exposed 
      to dynamic tensile strain for various time intervals in the presence of 
      interleukin (IL)-1beta. The regulation of the messenger RNA (mRNA) expression and 
      synthesis of MMPs and tissue inhibitors of MMPs (TIMPs) were examined by 
      end-point and real-time reverse transcriptase-polymerase chain reaction (RT-PCR) 
      as well as Western blot analysis. RESULTS: Fibrochondrocytes expressed mRNA for 
      MMP-2, -3, -7, -8, -9, -11, -13, -14, -16, -17, and -19 as well as TIMP-1, -2, 
      and -3, IL-1beta induced a significant (P<0.05) upregulation of mRNA for MMP-3, 
      -7, -8, -9, -13, -16, -17, and -19. The IL-1beta-stimulated upregulation of these 
      MMPs was significantly (P<0.05) abrogated by dynamic tensile strain. However, 
      MMP-2, -11, -14, and TIMPs were not affected by either IL-1beta or tensile 
      strain. Biomechanical strain also inhibited the IL-1beta-stimulated protein 
      synthesis of MMP-3, -7, -8, -9, -13, -16, and -17. Application of mechanical 
      strain for various time intervals during a 24-h incubation with IL-1beta showed 
      that the suppressive effects of mechanical signals are sustained. CONCLUSIONS: 
      The data provide evidence that biomechanical signals can downregulate the 
      catabolic activity of fibrocartilage cells in an inflammatory environment by 
      inhibiting the expression of a variety of MMPs. Furthermore, the 
      matrix-protective effects of biomechanical signals are sustained even in an 
      inflammatory environment.
FAU - Deschner, J
AU  - Deschner J
AD  - The Ohio State University, Oral Biology and Orthopedics, 4171 Postle Hall, 305 
      West 12th Avenue, Columbus, OH 43210, USA.
FAU - Rath-Deschner, B
AU  - Rath-Deschner B
FAU - Agarwal, S
AU  - Agarwal S
LA  - eng
GR  - R01 DE015399/DE/NIDCR NIH HHS/United States
GR  - DE15399/DE/NIDCR NIH HHS/United States
GR  - R01 AT000646/AT/NCCIH NIH HHS/United States
GR  - DE13799/DE/NIDCR NIH HHS/United States
GR  - AT000646/AT/NCCIH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20051114
PL  - England
TA  - Osteoarthritis Cartilage
JT  - Osteoarthritis and cartilage
JID - 9305697
RN  - 0 (Interleukin-1)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Chondrocytes/*enzymology/metabolism
MH  - Down-Regulation/physiology
MH  - Fibrocartilage/cytology/*enzymology/metabolism
MH  - Gene Expression Regulation, Enzymologic/*physiology
MH  - Interleukin-1/pharmacology
MH  - Matrix Metalloproteinases/*biosynthesis/genetics
MH  - Mechanotransduction, Cellular/*physiology
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Stress, Mechanical
MH  - Temporomandibular Joint/cytology/enzymology/metabolism
MH  - Tissue Inhibitor of Metalloproteinases/biosynthesis/genetics
PMC - PMC4948984
MID - NIHMS801995
EDAT- 2005/11/18 09:00
MHDA- 2006/05/04 09:00
PMCR- 2016/07/18
CRDT- 2005/11/18 09:00
PHST- 2005/02/04 00:00 [received]
PHST- 2005/09/14 00:00 [accepted]
PHST- 2005/11/18 09:00 [pubmed]
PHST- 2006/05/04 09:00 [medline]
PHST- 2005/11/18 09:00 [entrez]
PHST- 2016/07/18 00:00 [pmc-release]
AID - S1063-4584(05)00261-X [pii]
AID - 10.1016/j.joca.2005.09.005 [doi]
PST - ppublish
SO  - Osteoarthritis Cartilage. 2006 Mar;14(3):264-72. doi: 10.1016/j.joca.2005.09.005. 
      Epub 2005 Nov 14.