PMID- 16291224 OWN - NLM STAT- MEDLINE DCOM- 20060124 LR - 20231213 IS - 0076-6879 (Print) IS - 0076-6879 (Linking) VI - 396 DP - 2005 TI - The ESR method to determine nitric oxide in plants. PG - 84-92 AB - Nitric oxide (NO) plays an important role not only in animal system but also in plant system. We developed a method to detect NO free radical in plant system using electron spin resonance (ERS) spin trapping technique. The adduct (DETC)2-Fe2+-NO in plant can be extracted by organic solvent and then measured on an ESR spectrometer at room temperature (indirect method) or can be measured in live plant on an ESR spectrometer, but the water in the plant has to be partially dehydrated (direct method). FAU - Xu, Yangcang AU - Xu Y AD - Institute of Biophysics, Academia Sinica, Beijing, China. FAU - Cao, Yuanlin AU - Cao Y FAU - Tao, Yi AU - Tao Y FAU - Zhao, Baolu AU - Zhao B LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Methods Enzymol JT - Methods in enzymology JID - 0212271 RN - 31C4KY9ESH (Nitric Oxide) SB - IM MH - Electron Spin Resonance Spectroscopy/*methods MH - Nitric Oxide/*analysis MH - Glycine max/*chemistry EDAT- 2005/11/18 09:00 MHDA- 2006/01/25 09:00 CRDT- 2005/11/18 09:00 PHST- 2005/11/18 09:00 [pubmed] PHST- 2006/01/25 09:00 [medline] PHST- 2005/11/18 09:00 [entrez] AID - S0076-6879(05)96009-6 [pii] AID - 10.1016/S0076-6879(05)96009-6 [doi] PST - ppublish SO - Methods Enzymol. 2005;396:84-92. doi: 10.1016/S0076-6879(05)96009-6.