PMID- 16293215
OWN - NLM
STAT- MEDLINE
DCOM- 20060331
LR  - 20061115
IS  - 1672-7681 (Print)
IS  - 1672-7681 (Linking)
VI  - 1
IP  - 6
DP  - 2004 Dec
TI  - Specific antitumor effects of tumor vaccine produced by electrofusion between 
      osteosarcoma cell and dendritic cell in rats.
PG  - 454-60
AB  - Dendritic cells (DCs) are potent antigen-presenting cells capable of inducing 
      primary T-cell responses. Several immunotherapy treatment strategies involve 
      manipulation of DCs, both in vivo and ex vivo, to promote the immunogenic 
      presentation of tumor-associated antigens. In this study, an electrofusion 
      protocol was developed to induce fusion between osteosarcoma cells and allogeneic 
      bone marrow-derived DCs. Preimmunization with irradiated electrofusion products 
      was found to provide partial or complete protection from tumor challenge in the 
      UMR106 tumor model. Vaccinated survivors developed long immunological memory. The 
      therapeutic potential of this type of approach was suggested by the ability of 
      UMR106-DC electrofusion products which could induce tumor rejection in a 
      substantial percentage (60%) of hosts bearing pre-established tumor cells. These 
      results tended to indicate that treatment with electrofused tumor cells and 
      allogeneic DCs might be capable of inducing a potent antitumor response and could 
      conceivably be applied to a wide range of cancer indications for which 
      tumor-associated antigens have not been identified.
FAU - Yu, Zhe
AU  - Yu Z
AD  - Center of Orthopedic Surgery, Orthopedics Oncology Institute of Chinese PLA, 
      Tangdu Hospital, Fourth Military Medical University, Xi'an, Shanxi, China. 
      yuzhe19@fmmu.edu.cn
FAU - Fan, Qingyu
AU  - Fan Q
FAU - Hao, Xinbao
AU  - Hao X
FAU - Long, Hua
AU  - Long H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Cell Mol Immunol
JT  - Cellular & molecular immunology
JID - 101242872
RN  - 0 (Cancer Vaccines)
SB  - IM
MH  - Animals
MH  - Cancer Vaccines/*immunology
MH  - Cell Fusion
MH  - Cells, Cultured
MH  - Dendritic Cells/*cytology/*immunology
MH  - Immunotherapy
MH  - Male
MH  - Microscopy, Electron, Transmission
MH  - Osteosarcoma/*immunology/*pathology/therapy
MH  - Rats
MH  - Survival Rate
MH  - Xenograft Model Antitumor Assays
EDAT- 2005/11/19 09:00
MHDA- 2006/04/01 09:00
CRDT- 2005/11/19 09:00
PHST- 2005/11/19 09:00 [pubmed]
PHST- 2006/04/01 09:00 [medline]
PHST- 2005/11/19 09:00 [entrez]
PST - ppublish
SO  - Cell Mol Immunol. 2004 Dec;1(6):454-60.