PMID- 1629518
OWN - NLM
STAT- MEDLINE
DCOM- 19920818
LR  - 20220311
IS  - 0260-437X (Print)
IS  - 0260-437X (Linking)
VI  - 12
IP  - 3
DP  - 1992 Jun
TI  - The local lymph node assay: results of a final inter-laboratory validation under 
      field conditions.
PG  - 217-22
AB  - The local lymph node assay (LLNA) assesses the sensitizing activity of chemicals 
      by measurement of primary lymphocyte proliferation in lymph nodes draining the 
      site of application. In this final inter-laboratory study the consistency of LLNA 
      results between laboratories and with guinea pig maximization test (GPMT) data 
      was examined under 'field' conditions. Nine chemicals were evaluated 
      independently by each laboratory according to guidelines for test concentration 
      and vehicle selection developed during previous validation studies to ensure 
      assay optimization. Equivalent predictions of sensitization potential were 
      obtained by all laboratories for eight chemicals. Five of seven chemicals 
      identified as sensitizers in the GPMT were correctly identified in the LLNA--four 
      by all laboratories and 1 (4-chloroaniline) by one laboratory only--although in 
      this latter case, two other laboratories obtained clear dose responses, 
      suggestive of sensitization. The LLNA identified correctly those chemicals 
      predicted to be extreme or strong sensitizers in the GPMT. The remaining two 
      chemicals were non-sensitizers in the guinea pig and failed to elicit positive 
      proliferative responses in the LLNA. These data demonstrate that sensitivity and 
      reliability of the LLNA is retained when chemicals are evaluated independently, 
      and that it provides a reliable pre-screen for the identification of chemicals 
      with significant sensitization potential.
FAU - Scholes, E W
AU  - Scholes EW
AD  - Environmental Safety Laboratory, Unilever Research and Engineering, Sharnbrook, 
      Bedford, UK.
FAU - Basketter, D A
AU  - Basketter DA
FAU - Sarll, A E
AU  - Sarll AE
FAU - Kimber, I
AU  - Kimber I
FAU - Evans, C D
AU  - Evans CD
FAU - Miller, K
AU  - Miller K
FAU - Robbins, M C
AU  - Robbins MC
FAU - Harrison, P T
AU  - Harrison PT
FAU - Waite, S J
AU  - Waite SJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Appl Toxicol
JT  - Journal of applied toxicology : JAT
JID - 8109495
RN  - 0 (Irritants)
SB  - IM
MH  - Animals
MH  - Cell Division/drug effects
MH  - Female
MH  - Guinea Pigs
MH  - Irritants/toxicity
MH  - Lymph Nodes/*physiology
MH  - Lymphocytes/drug effects
MH  - Mice
MH  - Mice, Inbred CBA
EDAT- 1992/06/01 00:00
MHDA- 1992/06/01 00:01
CRDT- 1992/06/01 00:00
PHST- 1992/06/01 00:00 [pubmed]
PHST- 1992/06/01 00:01 [medline]
PHST- 1992/06/01 00:00 [entrez]
AID - 10.1002/jat.2550120312 [doi]
PST - ppublish
SO  - J Appl Toxicol. 1992 Jun;12(3):217-22. doi: 10.1002/jat.2550120312.