PMID- 16296333
OWN - NLM
STAT- MEDLINE
DCOM- 20060124
LR  - 20191210
IS  - 0047-1860 (Print)
IS  - 0047-1860 (Linking)
VI  - 53
IP  - 10
DP  - 2005 Oct
TI  - Nonrandom chromosomal numerical abnormality as a new molecular cytogenetic tumor 
      marker--a retrospective study of 60 gastric cancer cases.
PG  - 881-6
AB  - Chromosomal numerical abnormality (CNA) is one of the distinct characteristics of 
      human cancers, though the mechanisms and tumor specificity of this phenomenon 
      have not been adequately analyzed. Recently, we developed a new sensitive 
      fluorescence in situ hybridization (FISH) method that involves short-term 
      microwave (MW) treatment for hybridization. In this study, we applied this 
      modified FISH technique to investigate the CNA of 60 gastric cancer cases with a 
      panel of 18 chromosome-specific alpha-satellite probes (for chromosome 1-4, 6-12, 
      15-18, 20, X, and Y) and region-specific probes (c-myc, p53, and Her-2/neu) to 
      enumerate the respective chromosome numbers in interphase nuclei of 
      formalin-fixed paraffin-embedded sections. The numerical aberrations of 
      chromosome 1, 3, 8, 17, 20, and X were frequent regardless of histologic types, 
      whereas aberrations of chromosomes 10, 15, and 18 occurred less frequently 
      (p<0.001). From a histopathological standpoint, the mucocellular type of 
      carcinoma had stable CNA in comparison with the tubular type of carcinoma 
      (21.7+/-9.63% vs. 58.3+/-12.32%, p<0.001) and, of note, there was less extensive 
      CNA in female cases. A dramatic difference in patient outcome was detected 
      according to the involvement of chromosomes 3, 10, 11, 12, 17, and Y; cases with 
      CNA of these chromosomes had a worse prognosis (p<0.001). A two-step analysis of 
      the CNA of 6 chromosomes and locus specific gene abnormalities successfully 
      divided gastric cancer cases into those with a good outcome and those with a poor 
      outcome. This analysis allows one to more accurately predict prognosis than by 
      using a simple classification based on conventional clinicopathological 
      diagnosis.
FAU - Kitayama, Yasuhiko
AU  - Kitayama Y
AD  - Department of Pathology, Shizuoka Saiseikai General Hospital, Shizuoka 422-8527.
FAU - Sugimura, Haruhiko
AU  - Sugimura H
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - Japan
TA  - Rinsho Byori
JT  - Rinsho byori. The Japanese journal of clinical pathology
JID - 2984781R
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Biomarkers, Tumor
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human/*genetics
MH  - Female
MH  - Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Middle Aged
MH  - Predictive Value of Tests
MH  - Prognosis
MH  - Retrospective Studies
MH  - Stomach Neoplasms/*diagnosis/*genetics
EDAT- 2005/11/22 09:00
MHDA- 2006/01/25 09:00
CRDT- 2005/11/22 09:00
PHST- 2005/11/22 09:00 [pubmed]
PHST- 2006/01/25 09:00 [medline]
PHST- 2005/11/22 09:00 [entrez]
PST - ppublish
SO  - Rinsho Byori. 2005 Oct;53(10):881-6.