PMID- 1629670
OWN - NLM
STAT- MEDLINE
DCOM- 19920820
LR  - 20111117
IS  - 0022-1198 (Print)
IS  - 0022-1198 (Linking)
VI  - 37
IP  - 3
DP  - 1992 May
TI  - Polymerase chain reaction (PCR) amplification and human leukocyte antigen 
      (HLA)-DQ alpha oligonucleotide typing on biological evidence samples: casework 
      experience.
PG  - 700-26
AB  - The polymerase chain reaction (PCR) method of specific gene amplification was 
      used in casework to synthesize millions of copies of the polymorphic second exon 
      of the human leukocyte antigen (HLA)-DQ alpha (or DQA1) locus from a variety of 
      evidence samples. The HLA-DQ alpha allelic variants in the amplified 
      deoxyribonucleic acid (DNA) were determined in a rapid non-radioactive test by 
      hybridization to sequence-specific oligonucleotide probes in both the dot-blot 
      and reverse dot-blot formats. This genetic typing system has been subjected to 
      blind proficiency testing; the performance of this test in the analysis of 
      experimentally mixed samples was also evaluated. As of August 1990, over 250 
      cases have been tested and more than 2000 individual evidence (bloodstains, semen 
      stains, individual hairs, bone fragments, and tissue sections) and reference 
      samples have been analyzed. The first 198 of these cases are summarized in this 
      paper; in 65% of the cases with conclusive results a suspect was included, and in 
      35%, all suspects were excluded. Individual cases as well as some of the general 
      issues relating to forensic science analysis and this genetic typing system are 
      discussed. The high rate of exclusion reported here combined with the ability of 
      PCR to type old evidence samples suggests the relevance of this genetic test for 
      postconviction review; two cases in which the convicted suspect was excluded are 
      discussed.
FAU - Blake, E
AU  - Blake E
AD  - Forensic Science Associates, Richmond, CA.
FAU - Mihalovich, J
AU  - Mihalovich J
FAU - Higuchi, R
AU  - Higuchi R
FAU - Walsh, P S
AU  - Walsh PS
FAU - Erlich, H
AU  - Erlich H
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - J Forensic Sci
JT  - Journal of forensic sciences
JID - 0375370
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DQ alpha-Chains)
RN  - 0 (HLA-DQA1 antigen)
RN  - 0 (Oligonucleotide Probes)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adolescent
MH  - Alleles
MH  - Child
MH  - Child, Preschool
MH  - DNA/*analysis
MH  - Exons
MH  - Female
MH  - Forensic Medicine/*methods
MH  - Gene Amplification
MH  - HLA-DQ Antigens/*genetics
MH  - HLA-DQ alpha-Chains
MH  - Humans
MH  - Male
MH  - Nucleic Acid Hybridization
MH  - *Oligonucleotide Probes
MH  - *Polymerase Chain Reaction
MH  - Reproducibility of Results
EDAT- 1992/05/01 00:00
MHDA- 1992/05/01 00:01
CRDT- 1992/05/01 00:00
PHST- 1992/05/01 00:00 [pubmed]
PHST- 1992/05/01 00:01 [medline]
PHST- 1992/05/01 00:00 [entrez]
PST - ppublish
SO  - J Forensic Sci. 1992 May;37(3):700-26.