PMID- 16297090
OWN - NLM
STAT- MEDLINE
DCOM- 20051213
LR  - 20051121
IS  - 1075-122X (Print)
IS  - 1075-122X (Linking)
VI  - 11
IP  - 6
DP  - 2005 Nov-Dec
TI  - Numerical aberrations of chromosomes 1 and 7 by fluorescent in situ hybridization 
      and DNA ploidy analysis in breast cancer.
PG  - 448-53
AB  - The goals of this study were to detect the numerical alterations of chromosomes 1 
      and 7 in breast cancer and to correlate the findings with DNA ploidy status as 
      well as with parameters of prognostic significance. Fluorescence in situ 
      hybridization (FISH) with centromeric probes for chromosomes 1 and 7 and cellular 
      DNA content measurement by image analysis-based cytophotometry were applied on 
      interface nuclei from fresh tissue imprints of 59 breast ductal carcinomas. 
      Immunohistochemical stainings for estrogen receptor (ER), progesterone receptor 
      (PR), HER-2, p53, and Ki67 were performed on paraffin tumor sections. The 
      correlation between DNA ploidy and chromosomal aberrations revealed a significant 
      association between aneuploidy and aneusomy for both chromosomes 1 (p=0.002) and 
      7 (p=0.00001), however, a number of diploid tumors were found to be aneusomic, 
      especially for chromosome 1. Chromosome 7 polysomy was significantly associated 
      with a higher incidence of axillary lymph node metastasis (p=0.05), poorly 
      differentiated (grade III) tumors (p=0.03), negative ER and PR status (p=0.02 and 
      0.001, respectively), as well as p53 protein expression (p=0.05) and a higher 
      Ki67 labeling index (p=0.004). Chromosome 1 aneusomy was only related with HER-2 
      protein overexpression (p=0.05). No association between chromosome alterations 
      and tumor size was detected. In conclusion, the results of our study indicate 
      that the detection of numerical aberrations of chromosomes 1 and 7 by FISH seems 
      to be more sensitive than DNA ploidy status for the evaluation of abnormal 
      cellular DNA and chromosome 7 aneusomy characterizes tumors with aggressive 
      features and therefore might be a useful predictor of unfavorable biological 
      behavior in breast cancer.
FAU - Kapranos, Nikiforos
AU  - Kapranos N
AD  - Department of Molecular Pathology, MITERA Maternity and Surgical Center, Athens, 
      Greece. nkapranos@mitera.gr
FAU - Kounelis, Sophia
AU  - Kounelis S
FAU - Karantasis, Helen
AU  - Karantasis H
FAU - Kouri, Efi
AU  - Kouri E
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Breast J
JT  - The breast journal
JID - 9505539
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Receptors, Progesterone)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Breast Neoplasms/*genetics/pathology
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 1
MH  - *Chromosomes, Human, Pair 7
MH  - Female
MH  - Gene Expression Profiling
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Neoplasm Metastasis
MH  - Neoplasm Staging
MH  - Ploidies
MH  - Prognosis
MH  - Receptors, Estrogen/analysis
MH  - Receptors, Progesterone/analysis
EDAT- 2005/11/22 09:00
MHDA- 2005/12/15 09:00
CRDT- 2005/11/22 09:00
PHST- 2005/11/22 09:00 [pubmed]
PHST- 2005/12/15 09:00 [medline]
PHST- 2005/11/22 09:00 [entrez]
AID - TBJ123 [pii]
AID - 10.1111/j.1075-122X.2005.00123.x [doi]
PST - ppublish
SO  - Breast J. 2005 Nov-Dec;11(6):448-53. doi: 10.1111/j.1075-122X.2005.00123.x.