PMID- 16297473
OWN - NLM
STAT- MEDLINE
DCOM- 20061026
LR  - 20081121
IS  - 1874-1754 (Electronic)
IS  - 0167-5273 (Linking)
VI  - 112
IP  - 2
DP  - 2006 Sep 20
TI  - Angiotensin-II activates apoptosis, proliferation and protein synthesis in the 
      left heart ventricle of newborn albino rats.
PG  - 219-22
AB  - Angiotensin II (ANG-II) is a critical regulator of various signaling pathways 
      involved in growth and remodeling of the vascular, cardiac, and renal cells and 
      tissues. Although it contributes to several physiologic and pathologic events in 
      the cardiovascular system, its role in growth and differentiation of the newborn 
      heart is still unclear. We analyzed the effect of ANG-II treatment on apoptosis, 
      DNA synthesis, and nucleolar organizer regions (AgNORs) activity in newborn rat 
      myocardium. Injections of ANG-II for 5-days caused significant increase of the 
      3H-thymidine labeling index (M+/-m) in the myocardium of 7-day-old rats (from 
      6.95+/-0.32% to 8.53+/-0.22%, p<0.05). There was also significant increase in the 
      cross sectional surface area of cardiomyocytes (from 686+/-57 to 872+/-54 
      microm2, p<0.05), number of nucleoli (from 2.5+/-0.05 to 2.8+/-0.1, p<0.05), and 
      nucleolar surface area (from 2.6+/-0.09 to 3.2+/-0.22 microm2, p<0.05). These 
      changes were accompanied by significant increase in the apoptotic indices 
      analyzed by TDT-mediated dUTP-biotin nick end-labeling (TUNEL) (from 
      0.044+/-0.01% to 0.093+/-0.01%, p<0.05). Interestingly, we found no differences 
      in cell proliferation between the test and control animals after 21-45 days of 
      age, which were injected with ANG-II in the first postnatal week. However, the 
      area of cardiomyocytes and the number of nucleoli in 21-day-old rats continued to 
      increase significantly. Our results indicate that ANG-II modulates cardiac growth 
      during the neonatal period via stimulation of apoptosis, cell cycle events and 
      cellular growth of cardiomyocytes and that these effects can persist up to 15 
      days after injection of ANG-II has been completed.
FAU - Mel'nikova, Natalia P
AU  - Mel'nikova NP
AD  - Central Research Laboratory, Far-Eastern State Medical University, Khabarovsk, 
      Russia.
FAU - Timoshin, Sergey S
AU  - Timoshin SS
FAU - Jivotova, Elena Y
AU  - Jivotova EY
FAU - Pelliniemi, Lauri J
AU  - Pelliniemi LJ
FAU - Jokinen, Eero
AU  - Jokinen E
FAU - Abdelwahid, Eltyeb
AU  - Abdelwahid E
LA  - eng
PT  - Journal Article
DEP - 20051116
PL  - Netherlands
TA  - Int J Cardiol
JT  - International journal of cardiology
JID - 8200291
RN  - 0 (Antigens, Nuclear)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Vasoconstrictor Agents)
RN  - 0 (nucleolar organizer region associated proteins)
RN  - 11128-99-7 (Angiotensin II)
SB  - IM
MH  - Angiotensin II/*pharmacology/physiology
MH  - Animals
MH  - Animals, Newborn
MH  - Antigens, Nuclear/*biosynthesis/drug effects
MH  - Apoptosis/*drug effects
MH  - Cell Proliferation/*drug effects
MH  - Heart Ventricles/*drug effects
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Myocytes, Cardiac/metabolism
MH  - Nuclear Proteins/*biosynthesis/drug effects
MH  - Rats
MH  - Vasoconstrictor Agents/*pharmacology
MH  - Ventricular Function
EDAT- 2005/11/22 09:00
MHDA- 2006/10/27 09:00
CRDT- 2005/11/22 09:00
PHST- 2005/02/23 00:00 [received]
PHST- 2005/09/14 00:00 [revised]
PHST- 2005/09/21 00:00 [accepted]
PHST- 2005/11/22 09:00 [pubmed]
PHST- 2006/10/27 09:00 [medline]
PHST- 2005/11/22 09:00 [entrez]
AID - S0167-5273(05)01211-8 [pii]
AID - 10.1016/j.ijcard.2005.09.003 [doi]
PST - ppublish
SO  - Int J Cardiol. 2006 Sep 20;112(2):219-22. doi: 10.1016/j.ijcard.2005.09.003. Epub 
      2005 Nov 16.