PMID- 16298295
OWN - NLM
STAT- MEDLINE
DCOM- 20060302
LR  - 20071114
IS  - 1074-5521 (Print)
IS  - 1074-5521 (Linking)
VI  - 12
IP  - 11
DP  - 2005 Nov
TI  - Investigating beta-hydroxyenduracididine formation in the biosynthesis of the 
      mannopeptimycins.
PG  - 1163-8
AB  - The mannopeptimycins (MPPs) are potent glycopeptide antibiotics that contain both 
      D and L forms of the unique, arginine-derived amino acid 
      beta-hydroxyenduracididine (betahEnd). The product of the mppO gene in the MPP 
      biosynthetic cluster resembles several non-heme iron, 
      alpha-ketoglutarate-dependent oxygenases, such as VioC and clavaminate synthase. 
      The role of MppO in betahEnd biosynthesis was confirmed through inactivation of 
      mppO, which yielded a strain that produced dideoxy-MPPs, indicating that mppO is 
      essential for generating the beta-hydroxy functionality for both betahEnd 
      residues. Characterization in vitro of recombinant His6-MppO expressed in E. coli 
      revealed that MppO selectively hydroxylates the beta carbon of free 
      L-enduracididine.
FAU - Haltli, Brad
AU  - Haltli B
AD  - Natural Products Discovery, Wyeth Research, Pearl River, New York 10965, USA. 
      haltlib@wyeth.com
FAU - Tan, Ying
AU  - Tan Y
FAU - Magarvey, Nathan A
AU  - Magarvey NA
FAU - Wagenaar, Melissa
AU  - Wagenaar M
FAU - Yin, Xihou
AU  - Yin X
FAU - Greenstein, Michael
AU  - Greenstein M
FAU - Hucul, John A
AU  - Hucul JA
FAU - Zabriskie, T Mark
AU  - Zabriskie TM
LA  - eng
GR  - GM69320/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Chem Biol
JT  - Chemistry & biology
JID - 9500160
RN  - 0 (Amino Acids)
RN  - 0 (Glycopeptides)
RN  - 0 (Imidazolidines)
RN  - 0 (beta-hydroxyenduracididine)
RN  - 0 (mannopeptimycin)
SB  - IM
MH  - Amino Acids/*chemistry/*metabolism
MH  - Glycopeptides/*biosynthesis/chemistry
MH  - Imidazolidines/*chemistry/*metabolism
MH  - Mass Spectrometry
MH  - Molecular Sequence Data
MH  - Molecular Structure
MH  - Mutation/genetics
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Streptomyces/chemistry/*enzymology/genetics/metabolism
EDAT- 2005/11/22 09:00
MHDA- 2006/03/03 09:00
CRDT- 2005/11/22 09:00
PHST- 2005/05/31 00:00 [received]
PHST- 2005/09/01 00:00 [revised]
PHST- 2005/09/21 00:00 [accepted]
PHST- 2005/11/22 09:00 [pubmed]
PHST- 2006/03/03 09:00 [medline]
PHST- 2005/11/22 09:00 [entrez]
AID - S1074-5521(05)00307-8 [pii]
AID - 10.1016/j.chembiol.2005.09.013 [doi]
PST - ppublish
SO  - Chem Biol. 2005 Nov;12(11):1163-8. doi: 10.1016/j.chembiol.2005.09.013.