PMID- 16300664
OWN - NLM
STAT- MEDLINE
DCOM- 20051229
LR  - 20191210
IS  - 0105-6263 (Print)
IS  - 0105-6263 (Linking)
VI  - 28
IP  - 6
DP  - 2005 Dec
TI  - Low total normal motile count values are associated with increased sperm disomy 
      and diploidy rates in infertile patients.
PG  - 328-36
AB  - This study was undertaken to evaluate the possibility of identifying men at 
      increased risk of sperm aneuploidy and diploidy on the bases of specific cut-off 
      values of the total normal motile count (TNMC). Twenty-seven consecutive, 
      unselected male patients referred to our Unit were studied: 11 patients with 
      normal sperm parameters (group A) suffering from unexplained infertility and 16 
      infertile patients with abnormal sperm parameters (group B). Disomy rates for 
      chromosomes 1, 4, 8, 12, 18, X and Y were ascertained for each patient by means 
      of triple and double fluorescence in situ hybridization (FISH) experiments. Both 
      univariate and multivariate statistical analyses by principal component analysis 
      (PCA) were performed for comparisons between sperm aneuploidy rates and semen 
      quality (TNMC). TNMC scores in the two groups were significantly different (23.5 
      x 10(6) and 1.52 x 10(6), in groups A and B, respectively, p = 0.00002). In 
      general, higher sperm disomy rates were noted for all chromosomes in group B 
      compared with group A. Statistical significance was observed for disomy 1, total 
      disomy rate (3.36% vs. 1.38%), and diploidy (0.49% vs. 0.19%) (p < 0.01). For 
      disomy 4 and 8, differences resulted close to significance. PCA clearly showed 
      how independent variables were inter-related. Infertile men with TNMC < 2 x 10(6) 
      (male factor) were found to be at increased risk for sperm aneuploidy and 
      diploidy. Multivariate analysis by PCA resulted as a useful method to visualize 
      the information of the data sets on a bi-dimensional plot considering all the 
      patients and all the variables at the same time.
FAU - Bernardini, Luca M
AU  - Bernardini LM
AD  - Department of Obstetrics and Gynaecology, University of Genoa, Genoa, Italy.
FAU - Calogero, Aldo E
AU  - Calogero AE
FAU - Bottazzi, Cristina
AU  - Bottazzi C
FAU - Lanteri, Silvia
AU  - Lanteri S
FAU - Venturini, Pier Luigi
AU  - Venturini PL
FAU - Burrello, Nunziatina
AU  - Burrello N
FAU - De Palma, Adele
AU  - De Palma A
FAU - Conte, Nory
AU  - Conte N
FAU - Ragni, Nicola
AU  - Ragni N
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - England
TA  - Int J Androl
JT  - International journal of andrology
JID - 8000141
SB  - IM
MH  - Adult
MH  - *Aneuploidy
MH  - Chromosomes, Human, Pair 1
MH  - Chromosomes, Human, Pair 12
MH  - Chromosomes, Human, Pair 18
MH  - Chromosomes, Human, Pair 4
MH  - Chromosomes, Human, Pair 8
MH  - Chromosomes, Human, X
MH  - Chromosomes, Human, Y
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infertility, Male/*physiopathology
MH  - Male
MH  - Principal Component Analysis
MH  - *Sperm Count
MH  - *Sperm Motility
MH  - Spermatozoa/*abnormalities
EDAT- 2005/11/23 09:00
MHDA- 2005/12/31 09:00
CRDT- 2005/11/23 09:00
PHST- 2005/11/23 09:00 [pubmed]
PHST- 2005/12/31 09:00 [medline]
PHST- 2005/11/23 09:00 [entrez]
AID - IJA548 [pii]
AID - 10.1111/j.1365-2605.2005.00548.x [doi]
PST - ppublish
SO  - Int J Androl. 2005 Dec;28(6):328-36. doi: 10.1111/j.1365-2605.2005.00548.x.