PMID- 16301332
OWN - NLM
STAT- MEDLINE
DCOM- 20060106
LR  - 20181113
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 171
IP  - 4
DP  - 2005 Nov 21
TI  - Pleiotropic effects of FGFR1 on cell proliferation, survival, and migration in a 
      3D mammary epithelial cell model.
PG  - 663-73
AB  - Members of the fibroblast growth factor (FGF) family and the FGF receptors 
      (FGFRs) have been implicated in mediating various aspects of mammary gland 
      development and transformation. To elucidate the molecular mechanisms of FGFR1 
      action in a context that mimics polarized epithelial cells, we have developed an 
      in vitro three-dimensional HC11 mouse mammary epithelial cell culture model 
      expressing a drug-inducible FGFR1 (iFGFR1). Using this conditional model, iFGFR1 
      activation in these growth-arrested and polarized mammary acini initially led to 
      reinitiation of cell proliferation, increased survival of luminal cells, and loss 
      of cell polarity, resulting in the disruption of acinar structures characterized 
      by the absence of an empty lumen. iFGFR1 activation also resulted in a gain of 
      invasive properties and the induction of matrix metalloproteinase 3 (MMP-3), 
      causing the cleavage of E-cadherin and increased expression of smooth muscle 
      actin and vimentin. The addition of a pan MMP inhibitor abolished these 
      phenotypes but did not prevent the effects of iFGFR1 on cell proliferation or 
      survival.
FAU - Xian, Wa
AU  - Xian W
AD  - Department of Molecular and Cellular Biology, Baylor College of Medicine, 
      Houston, TX 77030, USA.
FAU - Schwertfeger, Kathryn L
AU  - Schwertfeger KL
FAU - Vargo-Gogola, Tracy
AU  - Vargo-Gogola T
FAU - Rosen, Jeffrey M
AU  - Rosen JM
LA  - eng
GR  - R37 CA016303/CA/NCI NIH HHS/United States
GR  - CA 97676-01/CA/NCI NIH HHS/United States
GR  - R01 CA016303/CA/NCI NIH HHS/United States
GR  - CA16303/CA/NCI NIH HHS/United States
GR  - F32 CA097676/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Cadherins)
RN  - 0 (RNA, Small Interfering)
RN  - EC 2.7.10.1 (Receptor, Fibroblast Growth Factor, Type 1)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Basement Membrane/metabolism
MH  - Cadherins/metabolism
MH  - Cell Line
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Dimerization
MH  - Epithelial Cells/*cytology
MH  - Epithelium/metabolism
MH  - Fluorescent Antibody Technique, Indirect
MH  - Imaging, Three-Dimensional
MH  - Immunoblotting
MH  - Mammary Glands, Animal/*cytology
MH  - Matrix Metalloproteinase 3/biosynthesis/metabolism
MH  - Mesoderm/metabolism
MH  - Mice
MH  - Microscopy, Confocal
MH  - Models, Biological
MH  - Phenotype
MH  - Plasmids/metabolism
MH  - RNA, Small Interfering/metabolism
MH  - Receptor, Fibroblast Growth Factor, Type 1/*physiology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction
MH  - Time Factors
MH  - Wound Healing
PMC - PMC2171554
EDAT- 2005/11/23 09:00
MHDA- 2006/01/07 09:00
PMCR- 2006/05/21
CRDT- 2005/11/23 09:00
PHST- 2005/11/23 09:00 [pubmed]
PHST- 2006/01/07 09:00 [medline]
PHST- 2005/11/23 09:00 [entrez]
PHST- 2006/05/21 00:00 [pmc-release]
AID - jcb.200505098 [pii]
AID - 200505098 [pii]
AID - 10.1083/jcb.200505098 [doi]
PST - ppublish
SO  - J Cell Biol. 2005 Nov 21;171(4):663-73. doi: 10.1083/jcb.200505098.