PMID- 16304675
OWN - NLM
STAT- MEDLINE
DCOM- 20060315
LR  - 20181113
IS  - 0733-2467 (Print)
IS  - 1520-6777 (Electronic)
IS  - 0733-2467 (Linking)
VI  - 25
IP  - 1
DP  - 2006
TI  - Muscarinic receptor transcript and protein density in hypertrophied and atrophied 
      rat urinary bladder.
PG  - 55-61
AB  - AIMS: Our previous studies showed that bladder hypertrophy shifts the muscarinic 
      receptor subtype mediating contraction from M(3) towards M(2) along with 
      increased M(2) and decreased M(3) protein concentration. We quantified mRNA for 
      M(1) through M(5) receptors to determine whether the changes in M(2) and M(3) 
      protein levels was due to changes in transcription. METHODS: Bladder hypertrophy 
      was induced by bladder outlet obstruction (BOO), major pelvic ganglion 
      electrocautery (DEN), and major pelvic ganglion decentralization (DEC). Bladder 
      atrophy was induced by ureteral diversion (DIV). Additional groups included 
      denervated and diverted (DEN-DIV), sham operated (SHAM), and normal (NOR) 
      controls. Transcripts were quantified using a multiplex ribonuclease protection 
      assay (RPA) and receptor protein density was determined by immunoprecipitation. 
      Receptor transcripts were expressed per unit total RNA. RESULTS: Although all 
      five receptor subtype transcripts were detected in all experimental groups, the 
      densities of M(1), M(4), and M(5) were much lower than for the M(2) and M(3) 
      subtype. There were more M(2) receptor transcripts than all the others, 
      consistent with M(2) protein determinations. M(2) transcripts were significantly 
      increased in DEN and BOO bladders. Surprisingly, M(3) transcripts were also 
      significantly increased in BOO. There was a significant correlation (r=0.98, 
      P<0.001) between protein density and transcript density for the M(2) but not the 
      M(3) receptor among the different experimental groups. CONCLUSIONS: Changes in 
      mRNA concentration are reflected by changes in protein density for the M(2) 
      receptor but not for the M(3) receptor. Extrapolation of functional effects from 
      transcript density data is invalid for M(3) mediated bladder contractions.
CI  - (c) 2005 Wiley-Liss, Inc.
FAU - Braverman, Alan S
AU  - Braverman AS
AD  - Department of Urology, Temple University School of Medicine, Philadelphia, 
      Pennsylvania 19140, USA.
FAU - Ruggieri, Michael R Sr
AU  - Ruggieri MR Sr
LA  - eng
GR  - R01 DK043333/DK/NIDDK NIH HHS/United States
GR  - R01 DK043333-10/DK/NIDDK NIH HHS/United States
GR  - R01 DK043333-11/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Neurourol Urodyn
JT  - Neurourology and urodynamics
JID - 8303326
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Muscarinic)
RN  - 0 (Ribosomal Proteins)
RN  - 0 (ribosomal protein L32)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - Animals
MH  - Atrophy
MH  - Denervation
MH  - Female
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/genetics
MH  - Hypertrophy
MH  - Nuclease Protection Assays
MH  - Proteins/metabolism
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Muscarinic/*genetics
MH  - Ribosomal Proteins/genetics
MH  - Urinary Bladder/*metabolism/pathology
MH  - Urinary Bladder Neck Obstruction/pathology
MH  - Urinary Diversion
PMC - PMC3277085
MID - NIHMS353505
EDAT- 2005/11/24 09:00
MHDA- 2006/03/16 09:00
PMCR- 2012/02/10
CRDT- 2005/11/24 09:00
PHST- 2005/11/24 09:00 [pubmed]
PHST- 2006/03/16 09:00 [medline]
PHST- 2005/11/24 09:00 [entrez]
PHST- 2012/02/10 00:00 [pmc-release]
AID - 10.1002/nau.20180 [doi]
PST - ppublish
SO  - Neurourol Urodyn. 2006;25(1):55-61. doi: 10.1002/nau.20180.