PMID- 16306213
OWN - NLM
STAT- MEDLINE
DCOM- 20060120
LR  - 20071115
IS  - 0031-3998 (Print)
IS  - 0031-3998 (Linking)
VI  - 58
IP  - 6
DP  - 2005 Dec
TI  - Induction of MCP1, CCR2, and iNOS expression in THP-1 macrophages by serum of 
      children late after Kawasaki disease.
PG  - 1306-10
AB  - Evidence of premature atherosclerosis late after Kawasaki disease (KD) is 
      accumulating. Given the potential roles of monocyte chemoattractant protein-1 
      (MCP-1), chemokine receptor CCR-2, and inducible nitric oxide synthase (iNOS) in 
      atherogenesis, we sought to determine whether serum obtained from children late 
      after KD would induce expression of these genes in macrophages in vitro. A total 
      of 79 subjects were studied, which comprised 57 KD patients, 33 of whom had 
      coronary aneurysms, and 22 age-matched controls. Expression of MCP-1, CCR2, and 
      iNOS mRNA in THP-1 macrophages in the presence of patient and control serum was 
      quantified as a ratio to beta-actin mRNA and expressed as a percentage of 
      control. MCP-1 expression was significantly increased in the presence of serum 
      from patients with coronary aneurysms. Expression of CCR2 and iNOS was 
      significantly increased when THP-1 macrophages were incubated with serum from 
      patients with and without coronary aneurysms. The magnitude of induction of 
      MCP-1, CCR2, and iNOS or in combinations correlated positively with serum 
      high-sensitivity C-reactive protein (hs-CRP), and low-density lipoprotein (LDL) 
      cholesterol levels and negatively with high-density lipoprotein (HDL) cholesterol 
      level. In conclusion, the serum of patients with a history of KD induces 
      expression of MCP-1, CCR2, and iNOS in THP-1 macrophages in vitro. Induction of 
      these genes in vivo may be related to chronic inflammation and may have important 
      implications for premature atherosclerosis.
FAU - Cheung, Yiu-Fai
AU  - Cheung YF
AD  - Department of Paediatrics and Adolescent Medicine, Grantham Hospital, The 
      University of Hong Kong, China. xfcheung@hkucc.hku.hk
FAU - O, Karmin
AU  - O K
FAU - Tam, Sidney C F
AU  - Tam SC
FAU - Siow, Yaw L
AU  - Siow YL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Pediatr Res
JT  - Pediatric research
JID - 0100714
RN  - 0 (CCL2 protein, human)
RN  - 0 (CCR2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Lipids)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
SB  - IM
MH  - Atherosclerosis/*genetics
MH  - Cells, Cultured
MH  - Chemokine CCL2/*genetics
MH  - Child
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Lipids/blood
MH  - Macrophages/metabolism
MH  - Male
MH  - Mucocutaneous Lymph Node Syndrome/*complications/genetics/immunology
MH  - Nitric Oxide Synthase Type II/*genetics
MH  - Receptors, CCR2
MH  - Receptors, Chemokine/*genetics
MH  - Serum/metabolism
MH  - Up-Regulation/*genetics
EDAT- 2005/11/25 09:00
MHDA- 2006/01/21 09:00
CRDT- 2005/11/25 09:00
PHST- 2005/11/25 09:00 [pubmed]
PHST- 2006/01/21 09:00 [medline]
PHST- 2005/11/25 09:00 [entrez]
AID - 58/6/1306 [pii]
AID - 10.1203/01.pdr.0000183360.79872.1c [doi]
PST - ppublish
SO  - Pediatr Res. 2005 Dec;58(6):1306-10. doi: 10.1203/01.pdr.0000183360.79872.1c.