PMID- 16309187
OWN - NLM
STAT- MEDLINE
DCOM- 20060105
LR  - 20131121
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 25
IP  - 6B
DP  - 2005 Nov-Dec
TI  - Unfiltered xenon light is useful for photodynamic therapy with acridine orange.
PG  - 3979-83
AB  - We have clarified that photodynamic therapy (PDT) with acridine orange (AO) 
      exerts a rapid and strong cytocidal effect on mouse osteosarcomas, both in vitro 
      and in vivo, and have sought to apply this therapy to patients with 
      musculoskeletal sarcomas, in order to reduce the surgical margin and obtain 
      better limb function after tumor resection in limb salvage surgery. Some clinical 
      studies have reported that the local recurrence rate after limb salvage surgery 
      in patients receiving PDT therapy was less than 10% and that the limb functions 
      recovered to nearly normal in these patients. For these basic and clinical 
      studies, we used a blue light beam filtered from a xenon lamp for the AO 
      excitation, because of its maximal absorption. However, the relationship between 
      the cytocidal effect of PDT and the wavelength or illuminance (lux) of the 
      excitation light in AO-PDT is unknown. Therefore, we investigated the cytocidal 
      effects of AO-PDT on mouse osteosarcoma cells using lights of various 
      illuminances and wavelengths from a xenon lamp. Our results revealed that, while 
      the blue and green filtered lights exerted cytocidal effects depending on their 
      illuminance, orange light exerted no such effect. Blue light showed the strongest 
      cytocidal effect under constant illuminance. However, unfiltered light with 10 
      times the illuminance of blue light yielded a much stronger cytocidal effect, 
      which was deduced not to be due to DNA injury by ultra-violet light or heat 
      generation by ultra-red light, since a xenon lamp emits little of either light. 
      Based on these results, we conclude that, for effective AO-PDT in clinical 
      practice, strong unfiltered light from a xenon lamp is more effective and 
      feasible than weak filtered blue light.
FAU - Ueda, Hideki
AU  - Ueda H
AD  - Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural 
      University of Medicine, Kyoto, Japan. ueda@cre-i.bias.ne.jp
FAU - Murata, Hiroaki
AU  - Murata H
FAU - Takeshita, Hideyuki
AU  - Takeshita H
FAU - Minami, Ginjiro
AU  - Minami G
FAU - Hashiguchi, Shin
AU  - Hashiguchi S
FAU - Kubo, Toshikazu
AU  - Kubo T
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 3H3U766W84 (Xenon)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*pharmacology
MH  - Animals
MH  - Bone Neoplasms/*drug therapy
MH  - Cell Line, Tumor
MH  - Light
MH  - Mice
MH  - Osteosarcoma/*drug therapy
MH  - Photochemotherapy/*methods
MH  - Xenon/*chemistry
EDAT- 2005/11/29 09:00
MHDA- 2006/01/06 09:00
CRDT- 2005/11/29 09:00
PHST- 2005/11/29 09:00 [pubmed]
PHST- 2006/01/06 09:00 [medline]
PHST- 2005/11/29 09:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 2005 Nov-Dec;25(6B):3979-83.