PMID- 16309551
OWN - NLM
STAT- MEDLINE
DCOM- 20060307
LR  - 20181113
IS  - 1471-230X (Electronic)
IS  - 1471-230X (Linking)
VI  - 5
DP  - 2005 Nov 25
TI  - Role of rapid urease test and histopathology in the diagnosis of Helicobacter 
      pylori infection in a developing country.
PG  - 38
AB  - BACKGROUND: The aim of this study was to determine the effect of commonly 
      self-prescribed proton pump inhibitors (PPI) on the results of rapid urease test 
      and histology for the diagnosis of H. pylori infection. METHODS: One hundred-nine 
      consecutive patients with dyspeptic symptoms attending the endoscopy suite were 
      enrolled in this study. Antrum biopsy specimens were collected at endoscopy for 
      the rapid urease test (Pronto Dry, Medical Instrument Corp, France) and 
      histopathology. Sensitivity, specificity, positive predictive value (PPV), 
      negative predictive value (NPV) and like-hood ratio of a positive and negative of 
      Pronto Dry test were compared against histology. The gold standard test for the 
      diagnosis of H. pylori infection was histopathology. RESULTS: Sixty-one percent 
      (66/109) patients were males with mean age of 43 +/- 14.1 years and age range 
      17-80 years. Fifty-two percent (57/109) were not on any medications while 48% 
      (52/109) used PPI before presentation to the outpatients. Pronto Dry was positive 
      in 40% (44/109) and negative in 60% (65/109). Histopathology was positive for H. 
      pylori in 57% (62/109) and negative in 43% (47/109). The sensitivity, 
      specificity, PPV, NPV and like-hood ratio of a positive and negative Pronto Dry 
      test with and without PPI were 43.3%, 86.4%, 81.3%, 3.18, 0.656 and 52.8% vs 
      71.9%, 80%, 82.1%, 69%, 3.59 and 0.35. CONCLUSION: This study shows that the 
      sensitivity, specificity, NPV and PPV of rapid urease test was reduced in 
      patients who are on PPI. The exclusive use of the rapid urease test for the 
      diagnosis of Helicobacter pylori cannot be recommended in patients with prior PPI 
      use.
FAU - Yakoob, Javed
AU  - Yakoob J
AD  - Department of Gastroenterology and Pathology, Aga Khan University Hospital, 
      Karachi, Pakistan. yakoobjaved@hotmail.com
FAU - Jafri, Wasim
AU  - Jafri W
FAU - Abid, Shahab
AU  - Abid S
FAU - Jafri, Nadim
AU  - Jafri N
FAU - Abbas, Zaigham
AU  - Abbas Z
FAU - Hamid, Saeed
AU  - Hamid S
FAU - Islam, Muhammad
AU  - Islam M
FAU - Anis, Kashif
AU  - Anis K
FAU - Shah, Hasnain Ali
AU  - Shah HA
FAU - Shaikh, Hizbullah
AU  - Shaikh H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20051125
PL  - England
TA  - BMC Gastroenterol
JT  - BMC gastroenterology
JID - 100968547
RN  - 0 (Proton Pump Inhibitors)
RN  - EC 3.5.1.5 (Urease)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Developing Countries
MH  - *Diagnostic Techniques, Digestive System
MH  - Female
MH  - Gastrointestinal Diseases/microbiology
MH  - Helicobacter Infections/complications/*diagnosis/*enzymology/pathology
MH  - Helicobacter pylori/*enzymology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Pakistan
MH  - *Proton Pump Inhibitors
MH  - Time Factors
MH  - Urease/*metabolism
PMC - PMC1316874
EDAT- 2005/11/29 09:00
MHDA- 2006/03/08 09:00
PMCR- 2005/11/25
CRDT- 2005/11/29 09:00
PHST- 2005/05/20 00:00 [received]
PHST- 2005/11/25 00:00 [accepted]
PHST- 2005/11/29 09:00 [pubmed]
PHST- 2006/03/08 09:00 [medline]
PHST- 2005/11/29 09:00 [entrez]
PHST- 2005/11/25 00:00 [pmc-release]
AID - 1471-230X-5-38 [pii]
AID - 10.1186/1471-230X-5-38 [doi]
PST - epublish
SO  - BMC Gastroenterol. 2005 Nov 25;5:38. doi: 10.1186/1471-230X-5-38.