PMID- 16310277
OWN - NLM
STAT- MEDLINE
DCOM- 20060720
LR  - 20181201
IS  - 0168-8278 (Print)
IS  - 0168-8278 (Linking)
VI  - 44
IP  - 3
DP  - 2006 Mar
TI  - Hepatic environment elicits monocyte differentiation into a dendritic cell subset 
      directing Th2 response.
PG  - 552-9
AB  - BACKGROUND/AIMS: Dendritic cells (DCs), which play a critical role during immune 
      response, could present alternative differentiation patterns depending on tissue 
      microenvironment. Our aim was to examine the influence of hepatic 
      microenvironment on human monocyte differentiation into DCs. METHODS: Cytology, 
      immunophenotyping, cytokine production and T-cell activation were analyzed in DCs 
      differentiated from human monocytes co-cultured with rat liver epithelial cells 
      (RLEC) or human cells from various tissue origins and compared to control DCs 
      obtained on plastic with GM-CSF/IL-4. RESULTS: RLEC environment promotes DC 
      differentiation in the presence of IL-4 without GM-CSF. These DCs evidence 
      similar expression of MHC-II, co-stimulatory and adhesion molecules than control 
      DCs, but distinct lineage markers defining a CD11c+/CD14+/CD123+ DC subset. This 
      phenotype is common to DCs from RLEC and human liver environment and differs from 
      that obtained with skin or intestine environments. Functionally, they produce 
      IL-10 but not IL-12p70 and favor IL-4/IL-10 secretion by T-cells rather than 
      IFN-gamma. CONCLUSIONS: Our results confirm that tissue niches modulate DC 
      differentiation and demonstrate that hepatic environment influences monocyte 
      differentiation into a DC subset directing Th2 response, a key data for 
      understanding the specialized immune response in liver. They also make RLEC 
      co-culture system useful for studying liver DC functions.
FAU - Cabillic, Florian
AU  - Cabillic F
AD  - INSERM U522, IFR140, Universite de Rennes 1, Hopital Pontchaillou, 35033 Rennes, 
      France.
FAU - Rougier, Nathalie
AU  - Rougier N
FAU - Basset, Christelle
AU  - Basset C
FAU - Lecouillard, Isabelle
AU  - Lecouillard I
FAU - Quelvennec, Erwann
AU  - Quelvennec E
FAU - Toujas, Louis
AU  - Toujas L
FAU - Guguen-Guillouzo, Christiane
AU  - Guguen-Guillouzo C
FAU - Corlu, Anne
AU  - Corlu A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050915
PL  - Netherlands
TA  - J Hepatol
JT  - Journal of hepatology
JID - 8503886
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - Dendritic Cells/*cytology/drug effects/metabolism
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Humans
MH  - *Immunity, Cellular
MH  - In Vitro Techniques
MH  - Interleukin-10/metabolism
MH  - Interleukin-4/pharmacology
MH  - Liver/cytology/*immunology/metabolism
MH  - Monocytes/*cytology/drug effects/metabolism
MH  - Phenotype
MH  - Rats
MH  - Th2 Cells/cytology/*immunology/metabolism
EDAT- 2005/11/29 09:00
MHDA- 2006/07/21 09:00
CRDT- 2005/11/29 09:00
PHST- 2005/03/18 00:00 [received]
PHST- 2005/07/05 00:00 [revised]
PHST- 2005/08/02 00:00 [accepted]
PHST- 2005/11/29 09:00 [pubmed]
PHST- 2006/07/21 09:00 [medline]
PHST- 2005/11/29 09:00 [entrez]
AID - S0168-8278(05)00561-1 [pii]
AID - 10.1016/j.jhep.2005.08.010 [doi]
PST - ppublish
SO  - J Hepatol. 2006 Mar;44(3):552-9. doi: 10.1016/j.jhep.2005.08.010. Epub 2005 Sep 
      15.