PMID- 16310816 OWN - NLM STAT- MEDLINE DCOM- 20060615 LR - 20111117 IS - 0034-5288 (Print) IS - 0034-5288 (Linking) VI - 81 IP - 1 DP - 2006 Aug TI - Changes in peripheral leukocytes enzymes activity and plasma metabolite concentrations in growing Holstein calves. PG - 19-23 AB - Plasma metabolite and immunoreactive insulin concentrations and activities of enzymes related to energy metabolism in peripheral leukocytes were measured in growing Holstein calves. A ratio of girth of abdomen divided by girth of thorax (A/T ratio) of calves was significantly elevated after weaning, and the A/T ratio maybe a good indicator to evaluate rumen development. Plasma glucose and free fatty acid concentrations were changed in calves accompanying change in feeding. Activities of lactate dehydrogenase with pyruvate as substrate (LDH-P) and hexokinase (HK) in cytosolic fractions of peripheral leukocytes decreased significantly after weaning the calves reflecting the change of energy source from milk replacer with high percentages of fat and glucose and lactose as absorbable carbohydrate to pelleted feed containing starch as less absorbable carbohydrate and roughage. Some peripheral leukocyte enzymes such as LDH and HK may be good indicators to evaluate changes in energy metabolism of growing calves. FAU - Arai, T AU - Arai T AD - Department of Veterinary Science, Nippon Veterinary and Animal Science University, 1-7-1 Kyonancho, Musashino, Tokyo 180-8602, Japan. tarai@nvau.ac.jp FAU - Tanaka, Y AU - Tanaka Y FAU - Urabe, S AU - Urabe S FAU - Kusaba, A AU - Kusaba A FAU - Tazaki, H AU - Tazaki H FAU - Ozawa, T AU - Ozawa T FAU - Kimura, N AU - Kimura N FAU - Jung, K K AU - Jung KK FAU - Waragaya, K AU - Waragaya K FAU - Yuyama, T AU - Yuyama T FAU - Haseba, Y AU - Haseba Y FAU - Imai, S AU - Imai S LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20051128 PL - England TA - Res Vet Sci JT - Research in veterinary science JID - 0401300 RN - 0 (Dietary Carbohydrates) RN - 0 (Insulin) RN - EC 1.1.1.27 (L-Lactate Dehydrogenase) RN - EC 2.7.1.1 (Hexokinase) SB - IM MH - Abdomen/anatomy & histology MH - Animal Feed MH - Animals MH - Biometry MH - Cattle/blood/*growth & development/*metabolism MH - Dietary Carbohydrates/metabolism MH - *Energy Metabolism MH - Hexokinase/metabolism MH - Insulin/*analysis MH - L-Lactate Dehydrogenase/metabolism MH - Leukocytes/*enzymology MH - Male MH - Plasma/metabolism MH - Thorax/anatomy & histology MH - Weaning EDAT- 2005/11/29 09:00 MHDA- 2006/06/16 09:00 CRDT- 2005/11/29 09:00 PHST- 2005/04/12 00:00 [received] PHST- 2005/10/03 00:00 [revised] PHST- 2005/10/05 00:00 [accepted] PHST- 2005/11/29 09:00 [pubmed] PHST- 2006/06/16 09:00 [medline] PHST- 2005/11/29 09:00 [entrez] AID - S0034-5288(05)00199-2 [pii] AID - 10.1016/j.rvsc.2005.10.002 [doi] PST - ppublish SO - Res Vet Sci. 2006 Aug;81(1):19-23. doi: 10.1016/j.rvsc.2005.10.002. Epub 2005 Nov 28.