PMID- 16311288
OWN - NLM
STAT- MEDLINE
DCOM- 20060605
LR  - 20220331
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 21
IP  - 4
DP  - 2006 Apr
TI  - Etoposide induces chromosomal abnormalities in mouse spermatocytes and stem cell 
      spermatogonia.
PG  - 888-95
AB  - BACKGROUND: Etoposide (ET) is a chemotherapeutic agent widely used in the 
      treatment of leukaemia, lymphomas and many solid tumours such as testicular and 
      ovarian cancers, all of which are common in patients of reproductive age. The 
      purpose of the study was to characterize the long-term effects of ET on male germ 
      cells using sperm fluorescence in situ hybridization (FISH) analyses. METHODS: 
      Chromosomal aberrations (partial duplications and deletions) and whole 
      chromosomal aneuploidies were detected in sperm of mice treated with a clinical 
      dose of ET. Semen samples were collected at 25 and 49 days after dosing to 
      investigate the effects of ET on meiotic pachytene cells and spermatogonial 
      stem-cells, respectively. RESULTS: ET treatment resulted in major increases in 
      the frequencies of sperm-carrying chromosomal aberrations in both meiotic 
      pachytene (27- to 578-fold) and spermatogonial stem-cells (8- to 16-fold), but 
      aneuploid sperm were induced only after treatment of meiotic cells (27-fold) with 
      no persistent effects in stem cells. CONCLUSION: These results show that ET may 
      have long-lasting effects on the frequencies of sperm with structural 
      aberrations. This has important implications for cancer patients undergoing 
      chemotherapy with ET because they may remain at higher risk for abnormal 
      reproductive outcomes long after the end of chemotherapy.
FAU - Marchetti, Francesco
AU  - Marchetti F
AD  - Biosciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA 
      94550, USA. marchetti2@llnl.gov
FAU - Pearson, Francesca S
AU  - Pearson FS
FAU - Bishop, Jack B
AU  - Bishop JB
FAU - Wyrobek, Andrew J
AU  - Wyrobek AJ
LA  - eng
GR  - Y01-ES-8016-5/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20051125
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 6PLQ3CP4P3 (Etoposide)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents, Phytogenic/*toxicity
MH  - Chromosome Aberrations/*chemically induced
MH  - Etoposide/*toxicity
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Meiosis
MH  - Mice
MH  - Spermatocytes/*drug effects
MH  - Spermatogonia/*drug effects
MH  - Stem Cells/drug effects
EDAT- 2005/11/29 09:00
MHDA- 2006/06/06 09:00
CRDT- 2005/11/29 09:00
PHST- 2005/11/29 09:00 [pubmed]
PHST- 2006/06/06 09:00 [medline]
PHST- 2005/11/29 09:00 [entrez]
AID - dei416 [pii]
AID - 10.1093/humrep/dei416 [doi]
PST - ppublish
SO  - Hum Reprod. 2006 Apr;21(4):888-95. doi: 10.1093/humrep/dei416. Epub 2005 Nov 25.