PMID- 16313356
OWN - NLM
STAT- MEDLINE
DCOM- 20060113
LR  - 20181113
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 116
IP  - 4
DP  - 2005 Dec
TI  - Leukotrienes modulate cytokine release from dendritic cells.
PG  - 418-28
AB  - Leukotriene B(4) (LTB(4)) and cysteinyl leukotrienes (CysLTs) are known as potent 
      mediators of inflammation, whereas their role in the regulation of adaptive 
      immunity remains poorly characterized. Dendritic cells (DCs) are specialized 
      antigen-presenting cells, uniquely capable to initiate primary immune responses. 
      We have found that zymosan, but not lipopolysaccharide (LPS) stimulates murine 
      bone marrow-derived dendritic cells (BM-DCs) to produce large amounts of CysLTs 
      and LTB(4) from endogenous substrates. A selective inhibitor of leukotriene 
      synthesis MK886 as well as an antagonist of the high affinity LTB(4) receptor 
      (BLT(1)) U-75302 slightly inhibited zymosan-, but not LPS-stimulated interleukin 
      (IL)-10 release from BM-DCs. In contrast, U-75302 increased zymosan-stimulated 
      release of IL-12 p40 by approximately 23%. Pre-treatment with transforming growth 
      factor-beta1 enhanced both stimulated leukotriene synthesis and the inhibitory 
      effect of U-75302 and MK886 on IL-10 release from DCs. Consistent with the 
      effects of leukotriene antagonists, exogenous LTB(4) enhanced LPS-stimulated 
      IL-10 release by approximately 39% and inhibited IL-12 p40 release by 
      approximately 22%. Both effects were mediated by the BLT(1) receptor. Ligands of 
      the high affinity CysLTs receptor (CysLT(1)), MK-571 and LTD(4) had little or no 
      effect on cytokine release. Agonists of the nuclear LTB(4) receptor peroxisome 
      proliferator-activated receptor-alpha, 8(S)-hydroxyeicosatetraenoic acid and 
      5,8,11,14-eicosatetraynoic acid, inhibited release of both IL-12 p40 and IL-10. 
      Our results indicate that both autocrine and paracrine leukotrienes may modulate 
      cytokine release from DCs, in a manner that is consistent with previously 
      reported T helper 2-polarizing effects of leukotrienes.
FAU - Jozefowski, Szczepan
AU  - Jozefowski S
AD  - Department of Immunology, Jagiellonian University School of Medicine, Krakow, 
      Poland. szjozefowski@poczta.onet.pl
FAU - Biedron, Rafal
AU  - Biedron R
FAU - Bobek, Malgorzata
AU  - Bobek M
FAU - Marcinkiewicz, Janusz
AU  - Marcinkiewicz J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Cytokines)
RN  - 0 (Leukotrienes)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Ltb4r1 protein, mouse)
RN  - 0 (Receptors, Leukotriene B4)
RN  - 0 (Receptors, Purinergic P2)
RN  - 0 (Tgfb1 protein, mouse)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 130068-27-8 (Interleukin-10)
RN  - 1HGW4DR56D (Leukotriene B4)
RN  - 9010-72-4 (Zymosan)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/immunology
MH  - Cells, Cultured
MH  - Cytokines/*metabolism
MH  - Dendritic Cells/*immunology
MH  - Interleukin-10/biosynthesis
MH  - Leukotriene B4/immunology
MH  - Leukotrienes/biosynthesis/*immunology
MH  - Lipopolysaccharides/immunology
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Receptors, Leukotriene B4/immunology
MH  - Receptors, Purinergic P2/immunology
MH  - Transforming Growth Factor beta/immunology
MH  - Transforming Growth Factor beta1
MH  - Zymosan/immunology
PMC - PMC1802435
EDAT- 2005/11/30 09:00
MHDA- 2006/01/18 09:00
PMCR- 2006/12/01
CRDT- 2005/11/30 09:00
PHST- 2005/11/30 09:00 [pubmed]
PHST- 2006/01/18 09:00 [medline]
PHST- 2005/11/30 09:00 [entrez]
PHST- 2006/12/01 00:00 [pmc-release]
AID - IMM2241 [pii]
AID - 10.1111/j.1365-2567.2005.02241.x [doi]
PST - ppublish
SO  - Immunology. 2005 Dec;116(4):418-28. doi: 10.1111/j.1365-2567.2005.02241.x.