PMID- 16313419
OWN - NLM
STAT- MEDLINE
DCOM- 20060306
LR  - 20131121
IS  - 1364-5072 (Print)
IS  - 1364-5072 (Linking)
VI  - 99
IP  - 6
DP  - 2005
TI  - Assessment of anaerobic wastewater treatment failure using terminal restriction 
      fragment length polymorphism analysis.
PG  - 1463-71
AB  - AIMS: The suitability of genetic fingerprinting to study the microbiological 
      basis of anaerobic bioreactor failure is investigated. METHODS AND RESULTS: Two 
      laboratory-scale anaerobic expanded granular sludge bed bioreactors, R1 and R2, 
      were used for the mesophilic (37 degrees C) treatment of high-strength [10 g 
      chemical oxygen demand (COD) l(-1)] synthetic industrial-like wastewater over a 
      100-day trial period. A successful start up was achieved by both bioreactors with 
      COD removal over 90%. Both reactors were operated under identical parameters; 
      however, increased organic loading during the trial induced a reduction in the 
      COD removal of R1, while R2 maintained satisfactory performance (COD removal 
      >90%) throughout the experiment. Specific methanogenic activity measurements of 
      biomass from both reactors indicated that the main route of methane production 
      was hydrogenotrophic methanogenesis. Terminal restriction fragment length 
      polymorphism (TRFLP) analysis was applied to the characterization of microbial 
      community dynamics within the system during the trial. The principal differences 
      between the two consortia analysed included an increased abundance of Thiovulum- 
      and Methanococcus-like organisms and uncultured Crenarchaeota in R1. CONCLUSIONS: 
      The results indicated that there was a microbiological basis for the deviation, 
      in terms of operational performance, of R1 and R2. SIGNIFICANCE AND IMPACT OF THE 
      STUDY: High-throughput fingerprinting techniques, such as TRFLP, have been 
      demonstrated as practically relevant for biomonitoring of anaerobic reactor 
      communities.
FAU - Scully, C
AU  - Scully C
AD  - Microbial Ecology Laboratory, Department of Microbiology and Environmental Change 
      Institute (ECI), National University of Ireland, Galway (NUI, Galway), University 
      Road, Galway, Ireland.
FAU - Collins, G
AU  - Collins G
FAU - O'Flaherty, V
AU  - O'Flaherty V
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (Industrial Waste)
RN  - 0 (Sewage)
RN  - 7YNJ3PO35Z (Hydrogen)
RN  - OP0UW79H66 (Methane)
SB  - IM
MH  - Anaerobiosis
MH  - Bacteria/*metabolism
MH  - Biodegradation, Environmental
MH  - Bioreactors
MH  - *Genes, Bacterial
MH  - Hydrogen/metabolism
MH  - Industrial Microbiology/*methods
MH  - *Industrial Waste
MH  - Methane/metabolism
MH  - *Polymorphism, Restriction Fragment Length
MH  - Sewage
MH  - *Waste Disposal, Fluid
EDAT- 2005/11/30 09:00
MHDA- 2006/03/07 09:00
CRDT- 2005/11/30 09:00
PHST- 2005/11/30 09:00 [pubmed]
PHST- 2006/03/07 09:00 [medline]
PHST- 2005/11/30 09:00 [entrez]
AID - JAM2743 [pii]
AID - 10.1111/j.1365-2672.2005.02743.x [doi]
PST - ppublish
SO  - J Appl Microbiol. 2005;99(6):1463-71. doi: 10.1111/j.1365-2672.2005.02743.x.