PMID- 16313780
OWN - NLM
STAT- MEDLINE
DCOM- 20070529
LR  - 20081121
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 85
IP  - 28
DP  - 2005 Jul 27
TI  - [Effects of interleukin-18 on asthmatic airway inflammation: experimental study 
      of guinea pig asthmatic model].
PG  - 1995-8
AB  - OBJECTIVE: To investigate the effects of Interleukin-18 (IL-18) on asthmatic 
      airway inflammation. METHODS: Thirty healthy adult male guinea pigs were randomly 
      divided into 3 equal groups: asthmatic model group (Group A, undergoing 
      intraperitoneally injection of ovalbumin (OVA) once and spraying of OVA aerosol 
      once a day for 5 days; control group (Group B), undergoing intraperitoneally 
      injection of OVA once and spraying of normal saline aerosol once a day for 5 
      days; and interleukin (IL)-18 intervention group (Group C, undergoing 
      intraperitoneally injection of OVA once and intraperitoneal injection of IL-18 on 
      the days 1, 3, 8. 10. 15, 17, and 19. Twenty-four hours after the final spraying 
      or IL-18 injection the bronchalveolar lavage fluid (BALF) of the left lungs were 
      obtained. HE staining was conducted to the sediment to examine the numbers of 
      eosinophils, neutrophils, and monocytes. ELISA was used to detect the Th1/Th2 
      cytokines in the BALF. The left lungs underwent pathological examination. 
      RESULTS: The number of EOS in BALF of Groups A was (98 +/- 58) x 10(6)/L, 
      significantly higher than those of Group B, (12 +/- 10) x 10(6)/L, and Group C, 
      (29 +/- 10) x 10(6)/L (P < 0.01 and P < 0.05). The numbers of neutrophils in the 
      BALF of Group A was (24 +/- 16) x 10(6)/L, significantly higher than those of 
      Group B and C [(9 +/- 7) x 10(6)/L and (10 +/- 5) x 10(6)/L respectively, both P 
      < 0.05]. The concentration of IFN-gamma and IL-2 in group A were both 
      significantly lower than those of Group B and Group C (P < 0.05 and P < 0.01). 
      The concentration of IL-4 in Group A was significantly higher than those of 
      Groups B and C (both P < 0.05). The concentration of IL-5 of Group A was 
      significantly higher than those of Group Bs and C (both P < 0.01). CONCLUSION: 
      IL-18 effectively inhibits asthmatic airway inflammation by regulating the 
      Th1/Th2 balance.
FAU - Chen, Xiang-qi
AU  - Chen XQ
AD  - Department of Respiratory Diseases, Union Hospital, Fujian Medical University, 
      Fuzhou 350001, China.
FAU - Lin, Ting-yan
AU  - Lin TY
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Interleukin-18)
RN  - 0 (Interleukin-5)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - Asthma/chemically induced/*drug therapy/pathology
MH  - Guinea Pigs
MH  - Interferon-gamma/analysis
MH  - Interleukin-18/*therapeutic use
MH  - Interleukin-4/analysis
MH  - Interleukin-5/analysis
MH  - Male
MH  - Ovalbumin
MH  - Random Allocation
MH  - Th1 Cells/*drug effects
MH  - Th2 Cells/*drug effects
EDAT- 2005/11/30 09:00
MHDA- 2007/05/30 09:00
CRDT- 2005/11/30 09:00
PHST- 2005/11/30 09:00 [pubmed]
PHST- 2007/05/30 09:00 [medline]
PHST- 2005/11/30 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2005 Jul 27;85(28):1995-8.