PMID- 16313938
OWN - NLM
STAT- MEDLINE
DCOM- 20060405
LR  - 20181201
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 346
IP  - 1
DP  - 2006 Mar 1
TI  - Molecular identification and phylogenetic analysis of baculoviruses from 
      Lepidoptera.
PG  - 180-93
AB  - PCR amplification of the highly conserved baculovirus genes late expression 
      factor 8 (lef-8), late expression factor 9 (lef-9) and polyhedrin/granulin 
      (polh/gran) combined with molecular phylogenetic analyses provide a powerful tool 
      to identify lepidopteran-specific baculoviruses and to study their diversity. In 
      the present investigation, we have improved the degenerate oligonucleotides and 
      corroborated the approach that was recently described by Lange et al. (Lange, M., 
      Wang, H., Zhihong, H., Jehle, J.A., 2004. Towards a molecular identification and 
      classification system of lepidopteran-specific baculoviruses. Virology 325, 
      36-47.). Baculovirus DNA was isolated from 71 uncharacterized historic 
      baculovirus samples, and partial gene sequences were amplified by using 
      gene-specific degenerate PCR primers. The obtained PCR products were directly 
      sequenced, and the deduced amino acid sequences were compiled and aligned with 
      published sequences of these target genes. A phylogenetic tree of 117 
      baculoviruses was inferred using maximum parsimony and distance methods. Based on 
      the comprehensive phylogenetic analysis of the partial lef-8, lef-9 and polh/gran 
      genes, we propose a phylogenetic species criterion for lepidopteran-specific 
      baculoviruses that uses the genetic distances of these genes for species 
      demarcation.
FAU - Jehle, Johannes A
AU  - Jehle JA
AD  - Laboratory for Biotechnological Crop Protection, Department of Phytopathology, 
      Agricultural Service Center Palatinate (DLR Rheinpfalz), Breitenweg 71, 67435 
      Neustadt an der Weinstrasse, Germany. Johannes.Jehle@dlr.rlp.de
FAU - Lange, Martin
AU  - Lange M
FAU - Wang, Hualin
AU  - Wang H
FAU - Hu, Zhihong
AU  - Hu Z
FAU - Wang, Yongjie
AU  - Wang Y
FAU - Hauschild, Rudiger
AU  - Hauschild R
LA  - eng
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PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051128
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (DNA, Viral)
RN  - 0 (Occlusion Body Matrix Proteins)
RN  - 0 (Viral Proteins)
RN  - 0 (Viral Structural Proteins)
RN  - 0 (late expression factor 8, Baculovirus)
RN  - 0 (polyhedrin protein, Nucleopolyhedrovirus)
SB  - IM
MH  - Animals
MH  - Baculoviridae/*classification/*genetics
MH  - DNA, Viral/analysis
MH  - Lepidoptera/*virology
MH  - Molecular Sequence Data
MH  - Occlusion Body Matrix Proteins
MH  - *Phylogeny
MH  - Sequence Analysis, DNA
MH  - Viral Proteins/genetics
MH  - Viral Structural Proteins
EDAT- 2005/11/30 09:00
MHDA- 2006/04/06 09:00
CRDT- 2005/11/30 09:00
PHST- 2005/08/30 00:00 [received]
PHST- 2005/09/27 00:00 [revised]
PHST- 2005/10/25 00:00 [accepted]
PHST- 2005/11/30 09:00 [pubmed]
PHST- 2006/04/06 09:00 [medline]
PHST- 2005/11/30 09:00 [entrez]
AID - S0042-6822(05)00727-0 [pii]
AID - 10.1016/j.virol.2005.10.032 [doi]
PST - ppublish
SO  - Virology. 2006 Mar 1;346(1):180-93. doi: 10.1016/j.virol.2005.10.032. Epub 2005 
      Nov 28.