PMID- 16315781
OWN - NLM
STAT- MEDLINE
DCOM- 20060126
LR  - 20161021
IS  - 1124-0490 (Print)
IS  - 1124-0490 (Linking)
VI  - 22
IP  - 3
DP  - 2005 Oct
TI  - Neurotrophin system activation in bronchoalveolar lavage fluid immune cells in 
      pulmonary sarcoidosis.
PG  - 186-94
AB  - BACKGROUND AND AIM OF THE STUDY: Pulmonary sarcoidosis is a chronic granulomatous 
      disease characterized by macrophage and CD4+ T-cell accumulation at the site of 
      inflammation. Analysis of the cytokine network has substantially improved 
      knowledge on immunopathogenesis of sarcoidosis. We hypothesize that neurotrophins 
      (NTs), nerve growth factor (NGF), brain derived neurotrophic factor (BDNF) and 
      NT-3, besides their importance in immune system activities, participate in 
      chronic inflammatory disorders and in repair processes. METHODS: The expression 
      of NTs and NT receptors was assessed in broncho alveolar lavage (BAL) 
      macrophages, CD4 and CD8 T-cells, from 10 patients with pulmonary sarcoidosis, 
      using molecular biology, Western blotting and immunocytochemistry. RESULTS: 
      Increased levels of NTs and of high affinity NT receptor (Trks) transcripts and 
      proteins in BAL macrophages, CD4+ and CD8+ T-cells from pulmonary sarcoidosis 
      patients were demonstrated in comparison with healthy controls. Contrarily to 
      healthy controls, in pulmonary sarcoidosis the expression of NGF was increased in 
      alveolar macrophages as well as NGF and BDNF in CD4+ and CD8+ T-cells. An 
      increased expression of TrkA, TrkB and TrkC receptors was also noticeable. 
      Furthermore, BDNF expression in alveolar macrophages and NT-3 expression in the 
      three different BAL immune cell populations investigated were induced during 
      sarcoidosis. A significant correlation was observed between CD4:CD8 ratio, 
      lymphocytosis, radiological stage and CD4 and CD8 NT expression. CONCLUSIONS: 
      These findings suggest that NTs are exaggeratedly expressed in BAL immune cells 
      in pulmonary sarcoidosis and may participate in the progression of disease 
      modulating immune cell functions.
FAU - Ricci, Alberto
AU  - Ricci A
AD  - Dipartimento di Scienze Cardiovascolari e Respiratorie, U.O.C. Pneumologia 
      Ospedale Sant'Andrea, Universita "La Sapienza" Roma, Italy. 
      alberto.ricci@uniroma1.it
FAU - Mariotta, Salvatore
AU  - Mariotta S
FAU - Saltini, Cesare
AU  - Saltini C
FAU - Falasca, Carlo
AU  - Falasca C
FAU - Giovagnoli, Maria Rosaria
AU  - Giovagnoli MR
FAU - Mannino, Francesco
AU  - Mannino F
FAU - Graziano, Paolo
AU  - Graziano P
FAU - Sciacchitano, Salvatore
AU  - Sciacchitano S
FAU - Amenta, Francesco
AU  - Amenta F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Sarcoidosis Vasc Diffuse Lung Dis
JT  - Sarcoidosis, vasculitis, and diffuse lung diseases : official journal of WASOG
JID - 9610928
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (DNA Primers)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
SB  - IM
MH  - Adult
MH  - Brain-Derived Neurotrophic Factor/genetics
MH  - Bronchoalveolar Lavage Fluid/chemistry/*immunology
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8-Positive T-Lymphocytes
MH  - DNA Primers
MH  - Female
MH  - Humans
MH  - Lymphocyte Count
MH  - Male
MH  - Middle Aged
MH  - Nerve Growth Factors/*genetics
MH  - Neurotrophin 3/genetics
MH  - Reference Values
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sarcoidosis, Pulmonary/genetics/*immunology
EDAT- 2005/12/01 09:00
MHDA- 2006/01/27 09:00
CRDT- 2005/12/01 09:00
PHST- 2005/12/01 09:00 [pubmed]
PHST- 2006/01/27 09:00 [medline]
PHST- 2005/12/01 09:00 [entrez]
PST - ppublish
SO  - Sarcoidosis Vasc Diffuse Lung Dis. 2005 Oct;22(3):186-94.