PMID- 16316536
OWN - NLM
STAT- MEDLINE
DCOM- 20100525
LR  - 20160607
IS  - 0578-1310 (Print)
IS  - 0578-1310 (Linking)
VI  - 43
IP  - 11
DP  - 2005 Nov
TI  - [Effects of immunoglobulin on the neuronal expression of IL-1beta and IL-1ra and 
      the neuronal death at hippocampus in rats with convulsion induced by 
      pentylenetetrazol].
PG  - 849-51
AB  - OBJECTIVE: To study the effects of immunoglobulin on the neuronal expression of 
      IL-1beta and IL-1ra and the neuronal death at hippocampus in rats with convulsion 
      induced by pentylenetetrazol. METHODS: The epilepsy model was established by 
      injecting intraperitoneally pentylenetetrazol (PTZ) into Wistar rats. Forty-five 
      rats were randomly divided into three groups, normal control group, PTZ plus 
      intravenous immunoglobulin (PTZ-IVIG); PTZ plus normal saline (PTZ-NS). Neuronal 
      death was assessed by light microscopy with the hematoxylin-eosin (HE) staining 
      and with in situ terminal deoxynucleotidyl transferase dUTP nick-end labeling 
      (TUNEL). IL-1beta and IL-1ra expressions were examined by histochemistry. 
      RESULTS: The ratio of IL-1beta/IL-1ra at hippocampal CA(1) region in PTZ-IVIG 
      group (0.5 +/- 0.1) was significantly lower than that in PTZ-NS group (1.9 +/- 
      0.5, t = 12.9, P < 0.05). Apoptotic cell numbers at the hippocampal CA(1) region 
      were significantly decreased in the PTZ-IVIG group, compared to PTZ-NS group (t = 
      27.1, P < 0.05). The numbers of positive cells were 16.4 +/- 3.3/1000 microm(2) 
      in the former and 41.7 +/- 3.5/1000 microm(2) in the latter. Necrotic cell 
      numbers at the hippocampal CA(1) region were significantly decreased in the 
      PTZ-IVIG group (19.0 +/- 2.6/1000 microm(2)), compared to PTZ-NS group (42.3 +/- 
      4.9/1000 microm(2), t = 20.9, P < 0.05). CONCLUSION: Immunoglobulin could inhibit 
      neuronal death induced by convulsion and its possible mechanism might be the 
      regulation of IL-1 system in neurons.
FAU - An, Ren-zhe
AU  - An RZ
AD  - Department of Pediatrics, Affiliated Hospital, Yanbian University Medical 
      College, Yanji 133000, China.
FAU - Yan, Guang-hai
AU  - Yan GH
FAU - Piao, Dong-ming
AU  - Piao DM
FAU - Yin, Yong-ri
AU  - Yin YR
FAU - Chi, Yong-xue
AU  - Chi YX
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Er Ke Za Zhi
JT  - Zhonghua er ke za zhi = Chinese journal of pediatrics
JID - 0417427
RN  - 0 (Immunoglobulins, Intravenous)
RN  - 0 (Interleukin 1 Receptor Antagonist Protein)
RN  - 0 (Interleukin-1beta)
RN  - WM5Z385K7T (Pentylenetetrazole)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Hippocampus/drug effects/*immunology/metabolism
MH  - Immunoglobulins, Intravenous/*pharmacology
MH  - Interleukin 1 Receptor Antagonist Protein/*metabolism
MH  - Interleukin-1beta/*metabolism
MH  - Neurons/*drug effects
MH  - Pentylenetetrazole/adverse effects
MH  - Rats
MH  - Rats, Wistar
MH  - Seizures/chemically induced/*immunology/metabolism
EDAT- 2005/12/01 09:00
MHDA- 2010/05/26 06:00
CRDT- 2005/12/01 09:00
PHST- 2005/12/01 09:00 [pubmed]
PHST- 2010/05/26 06:00 [medline]
PHST- 2005/12/01 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Er Ke Za Zhi. 2005 Nov;43(11):849-51.