PMID- 16320095
OWN - NLM
STAT- MEDLINE
DCOM- 20060607
LR  - 20200930
IS  - 0963-9292 (Print)
IS  - 0963-9292 (Linking)
VI  - 15
IP  - 1
DP  - 2006 Feb
TI  - Evaluation of the gill filament-based EROD assay in African sharptooth catfish 
      (Clarias gariepinus) as a monitoring tool for waterborne PAH-type contaminants.
PG  - 51-9
AB  - The ability of African sharptooth catfish (Clarias gariepinus) in inducing 
      cytochrome P-450 class 1A (CYP1A) and glutathione S-transferase (GST) biomarkers 
      was determined in liver and gill filaments after 4 days of waterborne exposure to 
      the polycyclic aromatic hydrocarbon, benzo[a]pyrene (B[a]P). Male (n=6) and 
      female (n=6) fish were exposed to B[a]P added to the water (30 microg/l) 
      corresponding to 5 mg/kg total body weight. Five female and six male fish, 
      exposed to acetone alone added to the water served as controls. The 
      7-ethoxyresurufin-O-deethylase (EROD) activity was measured in hepatic microsomes 
      and gill filaments. In addition, GST activity was determined in the hepatic 
      cytosolic fraction and fluorescent aromatic compounds (FACs) in bile and 
      biliverdin. Benzo[a]pyrene strongly induced EROD activities in gill filaments of 
      both sex. Levels of FACs per ml of bile were 17-fold higher in exposed fish 
      compared to the controls. Correlations between induction of EROD activities in 
      gill filaments and liver and between induction of EROD activities in gill 
      filaments and levels of biliary FACs metabolites were strong. GST activities in 
      the hepatic cytosolic fraction were similar amongst the treated and control 
      groups. This is the first report on studies determining EROD activities in gill 
      filaments and hepatic tissue, FACs in bile and GST in hepatic tissues of C. 
      gariepinus after waterborne exposure to B[a]P. The findings suggest that the gill 
      filament-based EROD assay in C. gariepinus can be used to monitor the pollution 
      of AhR agonists in aquatic ecosystems in eastern and southern African countries.
FAU - Mdegela, Robinson
AU  - Mdegela R
AD  - Department of Veterinary Medicine and Public Health, Faculty of Veterinary 
      Medicine, Sokoine University of Agriculture, P.O. Box 3021, Morogoro, Tanzania. 
      rmdegela@yahoo.com
FAU - Myburgh, Jan
AU  - Myburgh J
FAU - Correia, Dacia
AU  - Correia D
FAU - Braathen, Marte
AU  - Braathen M
FAU - Ejobi, Francis
AU  - Ejobi F
FAU - Botha, Christo
AU  - Botha C
FAU - Sandvik, Morten
AU  - Sandvik M
FAU - Skaare, Janneche Utne
AU  - Skaare JU
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Ecotoxicology
JT  - Ecotoxicology (London, England)
JID - 9885956
RN  - 0 (Biomarkers)
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
RN  - 0 (Water Pollutants, Chemical)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Animals
MH  - Bile/chemistry
MH  - Biological Assay
MH  - Biomarkers
MH  - Catfishes
MH  - Cytochrome P-450 CYP1A1/*metabolism
MH  - Environmental Monitoring
MH  - Female
MH  - Gills/*enzymology
MH  - Glutathione Transferase/metabolism
MH  - Liver/metabolism
MH  - Male
MH  - Polycyclic Aromatic Hydrocarbons/*analysis/toxicity
MH  - Water Pollutants, Chemical/*analysis/toxicity
EDAT- 2005/12/02 09:00
MHDA- 2006/06/08 09:00
CRDT- 2005/12/02 09:00
PHST- 2005/12/02 09:00 [pubmed]
PHST- 2006/06/08 09:00 [medline]
PHST- 2005/12/02 09:00 [entrez]
AID - 10.1007/s10646-005-0041-5 [doi]
PST - ppublish
SO  - Ecotoxicology. 2006 Feb;15(1):51-9. doi: 10.1007/s10646-005-0041-5.