PMID- 16321206
OWN - NLM
STAT- MEDLINE
DCOM- 20070608
LR  - 20051202
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 85
IP  - 32
DP  - 2005 Aug 24
TI  - [Effects of mechanical stress on expressions of monocyte chemoattractant 
      protein-1 and interleukin-8 of cultured human retinal pigment epithelial cells].
PG  - 2264-8
AB  - OBJECTIVE: To investigate the expressions of monocyte chemoattractant protein-1 
      (MCP-1) and interleukin-8 (IL-8) of cultured human retinal pigment epithelial 
      (hRPE) cells under mechanical stress in vitro, so as to mimic and understand the 
      role of these 2 inflammatory cytokines in the early stage of development of 
      primary retinal detachment. METHODS: Ferric oxide beads coated with collagen were 
      added to the culture plate containing wall-attaching hRPE cells and then the 
      cells were incubated and washed to remove the unbound beads. A magnet was put to 
      the culture plate to provide vertical magnetic force. Cytochalasin D (CD) was 
      added to the culture fluid to inhibit the phagocytizing, secreting, and moving 
      functions of the hRPE. Before the experiment and 15 min, 0.5 h, 1 h, 4 h, and 8 h 
      the beginning of experiment, the MCP-1 mRNA and IL-8 mRNA in the hRPE cells were 
      measured with reverse transcriptase polymerse chain reaction (RT-PCR), and the 
      MCP-1 and IL-8 protein expression in the supernatants was tested with 
      enzyme-linked immunosorbent assay (ELISA) kits. RESULTS: MCP-1 and IL-8 mRNA were 
      expressed at very low levels in the RPE cells and supernatant not exposed to 
      magnetic force. After exposure to magnetic force, both MCP-1 and IL-8 mRNA 
      demonstrated "double peaks" expression. Their first peak levels appeared within 
      0.5 h, being 3.30 ng/L +/- 0.12 ng/L and 1.88 ng/L +/- 0.08 ng/L respectively. 
      The first peaks of MCP-1 and IL-8 protein expression were within 1 h, being 
      552.05 ng/L +/- 7.64 ng/Land 236.67 ng/L +/- 14.30 ng/L respectively, and the 
      second peaks appeared about 4 hours later. After cytochalasin D pretreated, the 
      expression and secretion of both MCP-1 and IL-8 of the hRPE cells were 
      significantly decreased to the levels of 2.36 ng/L +/- 0.27 ng/L and 1.64 ng/L 
      +/- 0.08 ng/L, and 353.80 ng/L +/- 16.68 ng/L and 101.86 ng/L +/- 15.92 ng/L 
      respectively. CONCLUSION: Mechanical stress induces the RPE cells to express 
      MCP-1 and IL-8, and this effect was inhibited in part by pretreatment of CD, 
      indicating that the cytoskeleton may be involved in the effect and that these two 
      inflammatory cytokines take a part in the early stage of development of primary 
      retinal detachment.
FAU - Zhang, Xiao-guang
AU  - Zhang XG
AD  - Eye Institute of the People's Liberation Army, Xijing Hospital, Fourth Military 
      Medical University, Xi'an 710032, China.
FAU - Hui, Yan-nian
AU  - Hui YN
FAU - Han, Quan-hong
AU  - Han QH
FAU - Hou, Xu
AU  - Hou X
FAU - Chen, Li-jun
AU  - Chen LJ
FAU - Ma, Ji-xian
AU  - Ma JX
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-8)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis/genetics
MH  - Humans
MH  - Interleukin-8/*biosynthesis/genetics
MH  - Magnetics
MH  - Pigment Epithelium of Eye/cytology/*metabolism
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Stress, Mechanical
EDAT- 2005/12/03 09:00
MHDA- 2007/06/09 09:00
CRDT- 2005/12/03 09:00
PHST- 2005/12/03 09:00 [pubmed]
PHST- 2007/06/09 09:00 [medline]
PHST- 2005/12/03 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2005 Aug 24;85(32):2264-8.